| 重组碱性成纤维细胞生长因子ELISA检测方法的建立 |
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| 引用本文： | 向军俭,吴彤,杨红宇,林剑.重组碱性成纤维细胞生长因子ELISA检测方法的建立[J].暨南大学学报,1993(1). |
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| 作者姓名： | 向军俭 吴彤 杨红宇 林剑 |
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| 作者单位： | 暨南大学生物学系，暨南大学生物学系，暨南大学生物学系，暨南大学生物学系 |
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| 基金项目： | 国家科委85攻关项目，国家自然科学基金资助项目部分内容 |
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| 摘 要： | 小鼠或家克经重组bFGF免疫后产生高滴度的抗血清,用此血清建立了间接ELISA检测方法,检测bFGF的灵敏度达10ng/ml.
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| 关 键 词： | 重组碱性成纤维细胞生长因子 酶联免疫吸附试验 抗碱性成纤维细胞生长因子抗体 |
ESTABLISHMENT OF ELISA METHOD FOR ASSAY OF RECOMBINANT FIBROBLAST GROWTH FACTOR(rbFGF) |
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| Xiang Junjian Wu Tong Yang Hongyu Lin Jian.ESTABLISHMENT OF ELISA METHOD FOR ASSAY OF RECOMBINANT FIBROBLAST GROWTH FACTOR(rbFGF)[J].Journal of Jinan University(Natural Science & Medicine Edition),1993(1). |
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| Authors: | Xiang Junjian Wu Tong Yang Hongyu Lin Jian |
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| Institution: | Dept. of Biology |
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| Abstract: | Mouse and rabbit antiserum of recombinant bFGF was produced. The titer was about 1:2000 (ELISA). A specific and simple indirect ELISA method was established with these antibodies. The sensitivity of this method was about 10 ng/ml. |
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| Keywords: | rbFGF ELISA anti-bFGF antibody |
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