蛇足石杉内生真菌 TL 的分离鉴定与药敏研究

从蛇足石杉植株中分离出内生真菌，通过马铃薯培养基（PDA）培养菌株，利用三点接种法分离纯化，筛选出一株优势菌株TL作为供试菌株通过菌落形态和培养特性观察以及显微摄影技术进行形态观察，对照《真菌鉴定手册》，经鉴定为半知菌亚门( Amon imperfect fungi )丝孢纲（Hyphomycetes）丛梗孢科(Moniliaceae)单端孢属(Trichothecium Link ex Fries)通过单因素试验与正交试验相结合，最终发现对供试菌株具有最佳抑菌效果的药物浓度组合

Isolation and Identification of Endophytic Fungi TL from Huperzia Serrata and Antibiotics Sensitivity Research

Endophytic fungi was isolated from the plant Huperzia serrata.The strains was cultured through the medium of potato (PDA) and purified by three-point inoculation.Finally a dominant strain named TL was seleted as the tested strains.Through the observation of colony morphology and culture characteristics and microscopic morphology by photographic technology,TL was identified as Amon imperfect fungi,Hyphomycetes Moniliaceae,Trichothecium Link ex Fries according to the Identification Handbook of Fungus.Through the single-factor experiment and orthogonal test,the authors found the best combination of effective drug concentration with the best inhibitory effect on the tested strains.