| 拟南芥FIE基因特异片段的克隆及序列测定 |
| |
| 引用本文： | 郑天然,李荣田,李海英,刘丽萍,郭德栋.拟南芥FIE基因特异片段的克隆及序列测定[J].黑龙江大学自然科学学报,2004,21(3):132-136. |
| |
| 作者姓名： | 郑天然 李荣田 李海英 刘丽萍 郭德栋 |
| |
| 作者单位： | 黑龙江大学,生命科学学院,黑龙江,哈尔滨,150080;黑龙江大学,生命科学学院,黑龙江,哈尔滨,150080;黑龙江大学,生命科学学院,黑龙江,哈尔滨,150080;黑龙江大学,生命科学学院,黑龙江,哈尔滨,150080;黑龙江大学,生命科学学院,黑龙江,哈尔滨,150080 |
| |
| 基金项目： | 国家转基因植物研究与产业化开发专项资助项目(JYD3-A-2402)，黑龙江省自然科学基金重点资助项目(ZJN-03-6) |
| |
| 摘 要： | 根据拟南芥FIE基因的序列设计PCR引物,以拟南芥基因组DNA为模板,通过PCR反应扩增FIE基因特异性片段,扩增产物经琼脂糖凝胶电泳分析,长为1633bp,与预期结果相符。将该基因片段定向克隆到pGEM-T载体上,得到重组质粒p1633,酶切鉴定及测序分析结果表明该克隆为拟南芥FIE基因特异片段,与拟南芥FIE基因比较同源性达99.8％,可以作为探针使用。
|
| 关 键 词： | 拟南芥 FIE基因 PCR 同源性 |
| 文章编号： | 1001-7011(2004)03-0132-05 |
| 修稿时间： | 2004年2月26日 |
Cloning and sequencing of specific fragment of Arabidopsis thaliana FIE gene |
| |
| ZHENG Tian-ran,LI Rong-tian,LI Hai-ying,LIU Li-ping,GUO De-dong.Cloning and sequencing of specific fragment of Arabidopsis thaliana FIE gene[J].Journal of Natural Science of Heilongjiang University,2004,21(3):132-136. |
| |
| Authors: | ZHENG Tian-ran LI Rong-tian LI Hai-ying LIU Li-ping GUO De-dong |
| |
| Abstract: | According to the sequence of Arabidopsis thaliana FIE gene, PCR primers were designed. FIE specific fragment was amplified on the model of Arabidopsis thaliana DNA. The product was 1633 bp in length by gelose gel electrophoresis and same to the prospective result. Linked to pGEM-T Vector, the recombinant plasmid was p1633. Restriction analysis and sequence analysis had proved that the clone product was the specific fragment of Arabidopsis thaliana FIE gene. Compared with Arabidopsis thaliana FIE gene, the cloned FIE fragment shared 99.8% homology at nucleotide level. The specific fragment can be used as probes. |
| |
| Keywords: | Arabidopsis thaliana FIE gene PCR homology |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
