| 改良QuEChERS-高效液相色谱法检测柑橘中的多菌灵 |
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| 引用本文： | 周晓洁,赵良忠,夏湘,尹乐斌,王丽,周小虎. 改良QuEChERS-高效液相色谱法检测柑橘中的多菌灵[J]. 邵阳学院学报(自然科学版), 2014, 0(2): 74-78 |
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| 作者姓名： | 周晓洁 赵良忠 夏湘 尹乐斌 王丽 周小虎 |
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| 作者单位： | 邵阳学院生物与化学工程系,湖南邵阳422000 |
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| 基金项目： | 邵阳学院研究生创新项目（CX2013XY014） |
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| 摘 要： | 建立了柑橘中多菌灵残留量的改良QuEChERS-高效液相色谱快速检测方法.样品用10%醋酸乙腈溶液提取、醋酸钠和无水硫酸镁盐析后,取上清液,加入N-丙基乙二胺(PSA)与石墨化炭黑(GCB)吸附剂净化,用高效液相色谱-UV检测器测定,外标法定量.多菌灵在0.05~5.0mg/L(r=0.9983)范围内与峰面积呈良好线性关系;方法检出限为0.01mg/kg;回收率为72.0%~99.0%;相对标准偏差小于4%.该方法操作简便、准确、快速、成本较低,适用于柑橘中多菌灵残留量的测定.
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| 关 键 词： | QuEChERS 高效液相色谱 柑橘 多菌灵 |
Determination of Carbendazim Residue in Citrus by High Performance Liquid Chromatography with Modified QuEChERS |
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| ZHOU Xiao-jie,ZHAO Liang-zhong,XIA Xiang,YIN Le-bin,WANG Li,ZHOU Xiao-hu. Determination of Carbendazim Residue in Citrus by High Performance Liquid Chromatography with Modified QuEChERS[J]. Journal of Shaoyang University(Natural Science Edition), 2014, 0(2): 74-78 |
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| Authors: | ZHOU Xiao-jie ZHAO Liang-zhong XIA Xiang YIN Le-bin WANG Li ZHOU Xiao-hu |
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| Affiliation: | ( Department of Biological and Chemical Engineering, Shaoyang University, Shaoyang, Hunan 422000, China) |
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| Abstract: | A developed method for determination of carbendazim in citrus by QuEChERS-HPLC was established. Samples were extracted with acetonitrile containing 10% acetic acid and salted out by anhydrous MgSO4 and CH3COONa. Purifying by Npropyl ethylenediamine（PSA）cartridge and graphitized carbon black（GCB）,the carbendazim in the solution was determined by HPLC-UV. The linear range is 0.05-5.0mg/L（r=0. 9983） and the detection limit was 0.01 mg/kg. The recovery was from72% to 99%,relative standard deviation was less than 4%. The proposed method was simple,accurate,quick,low-cost and ap plicable for the determination of carbendazim residue in Citrus. |
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| Keywords: | QuEChERS high performance liquid chromatographic（HPLC） citrus carbendazim |
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