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| 沙葱多糖的SephadexG-100凝胶柱纯化和分子量的测定 | |
| 引用本文： | 扈瑞平.沙葱多糖的SephadexG-100凝胶柱纯化和分子量的测定[J].科学技术与工程,2011,11(12):2780-2783. |
| 作者姓名： | 扈瑞平 |
| 作者单位： | 1. 内蒙古医学院基础医学院,呼和浩特,010059;内蒙古农业大学动物科学与医学院,呼和浩特,010018 2. 内蒙古师范大学生命科学与技术学院,呼和浩特,010022 3. 内蒙古农业大学动物科学与医学院,呼和浩特,010018 4. 内蒙古医学院基础医学院,呼和浩特,010059 |
| 基金项目： | 国家自然科学基金(30960253)资助 |
| 摘 要： | 对通过热水浸提、三氯乙酸除蛋白和活性炭脱色后的沙葱粗多糖,采用SephadexG—100凝胶柱层析纯化,收集合并单一峰溶液命名为SCSP,并对纯化产物SCSP进行相对分子质量的测定。结果表明,SCSP凝胶柱层析洗脱曲线为单一狭窄对称峰,说明为均一组分多糖;SCSP的相对分子质量为5.89×104克/摩尔。 |
| 关 键 词： | 沙葱多糖 SephadexG—100凝胶柱层析 分子量 |
| 收稿时间： | 2011/1/21 0:00:00 |
| 修稿时间： | 2/22/2011 6:31:41 PM |
Studies on purified with SephadexG-100 column chromatography and molecular weight measured of Allium mongolium Regel Polysaccharides |
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| huruiping.Studies on purified with SephadexG-100 column chromatography and molecular weight measured of Allium mongolium Regel Polysaccharides[J].Science Technology and Engineering,2011,11(12):2780-2783. | |
| Authors: | huruiping |
| Institution: | HU Rui-ping1,2,DU Ling3,AO Chang-jin2*,ZHANG Xin-fu2,DENG Feng1(Basic Curriculum Department of Inner Mongolia Medical College1,Hohhot 010059,P.R.China,College of Animal Science and Animal Medicine of Inner Mongolia Agricultural University2,Hohhot 010018,College of life Science and Technology of Inner Mongolia Normal University3,Hohhot 010022,P.R.China) |
| Abstract: | To rough sand onion polysaccharides, which by hot water extraction, trichloramine acetic except protein and activated carbon decoloring , adopting sand onion SephadexG - 100 gel column chromatography purification, collect merger single submmit solution named SCSP, and the purification product SCSP for molecular weight determination. The results show that SCSP gel column chromatography elution curve for a single narrow symmetrical peak, explain for homogeneous components polysaccharide; SCSP relative molecular mass for 5.89 x 104 D. |
| Keywords: | Allium Mongolicum Regel polysaccharide SephadexG-100 column chromatography molecular weight measure |
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