层层组装纳米金/天冬氨酸传感器对尿酸的电化学性质研究

采用层层组装法制备了金和天冬氨酸复合膜传感器.用循环伏安法(CV)和脉冲伏安法(DPV)等研究了尿酸在该传感器上的电化学行为.结果表明,在PBS 7.0作为支持电解质的条件下,尿酸在该组装传感器上的氧化峰的峰电流是裸电极传感器上的6.5倍.优化条件下,用DPV对尿酸进行了测定,在尿酸浓度为4.0×10-7~1.0×10-4 mol/L范围内浓度与尿酸的氧化峰电流具有良好的线性关系,线性方程为:I(μA)=0.010-0.022 C(μmol/L),相关系数为0.998.检出限(RSN=3)为1.0×10-7 mol/L.该方法用于实际尿样的测定,回收率为99.4%~104.1%.

Electrochemical Properties of Uric Acid on the Gold-aspartic Acid Sensor Assembled via a Layer-by-layer Method

Gold-aspartic acid composite film modified electrode was fabricated via a layer by layer technique.The electrochemical properties of uric acid(UA)on the sensor were investigated by cyclic voltammetry(CV)and differential pulse voltammetry(DPV).The results demonstrated that the oxidation peak current of UA on the sensor was 6.5times than that of bare electrode when PBS 7.0acting as the supporting electrolyte.Under optimum conditions,the oxidation peak current of UA had a linear relationship as a function of its concentration ranging from 4.0×10-7 to 1.0×10-4 mol/L.The linear equation wasI(μA)= 0.010-0.022 C(μmol/L).The correlation coefficient and detection limit(RSN=3)were 0.998 and 1.0×10-7 mol/L,respectively.The recovery efficiency was 99.4% ~ 104.1% when the method was used for actual urine sample detection.