Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface |
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Authors: | Langchong He Guangde Yang Xindu Geng |
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Institution: | (1) Institute of Modern Separation Science, Korthwest University, 710069 Xi’an, China;(2) College of Pharmacy, Xi’an Medical University, 710061 Xi’an, China |
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Abstract: | A new cell membrane preparation. silica carrier cell membrane (CCM), has been presented, in which cell membrane was immobilized
on silica which acted as carrier by the absorption. The surface characteristics of the silica CCM have been tested by a scanning
electron microscope and surface energy spectrometer. Comparison has been made of the changeable rule of enzymatic activity
of rabbit red cell membrane in three states: the silica CCM, the suspension and the precipitation cell membranes, with temperature
and time. The result showed that the silica CCM still keeps the same enzymatic activity as the other two states of cell membranes.
The interactions between calcium antagonists and rabbi red and cardiac muscle cell membranes, and the stereoscopic interactions
between dihydropridines and rabbit cerebellar cell membrane have been investigated by the chromatographic system of the silica
CCM as a stationary phase. From the foregoing results, the chromatographic parameters obtained from the system would be expected
to indicate the special interaction and enantioselectivity between drugs and membrane receptorsin vivo. |
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Keywords: | cell membrane preparation silica CCM enzymatic activity affinity chromatography enantiomers |
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