人肺癌裸鼠常位移植实验研究

将高转移人肺巨细胞癌和低转移人肺腺癌常位移植于裸鼠肺内,其移植成功率分别为74％和56％,肺内移植瘤仍保持人原发肿瘤的病理组织学形态,其肉眼类型可呈肺门肿块,周围结节和弥散浸润型,与人类原发肺癌亦相似,而与局限膨胀生长或较少浸润的裸鼠皮下移植瘤有明显区别。肺内常位移植瘤较广泛地浸润胸部脏器,特别应当指出的是低转移肺腺癌细胞肺内移植其淋巴结转移率可达56％,甚至高于该癌系在beige裸鼠体内的淋巴结转移率。说明常位移植有利于恶性肿瘤表达其浸润转移表型。常位移植之肿瘤生物学行为更接近人体内原发性肿瘤的生物学行为,将成为研究人类肿瘤特性和实验治疗的更为适宜的实验模型。研究裸鼠皮下移植瘤和常位移植瘤基因表达的差异亦将为肿瘤浸润转移分子生物学基础的揭示作出贡献

EXPERIMENTAL STUDIES ON ORTHOTOPIC XENOTRANS-PLANTATION OF HUMAN PULMONARY CARCINOMAS IN NUDE MICE

A highly metastatic giant cell carcinoma (PG) and a poorly metastatic adenocarcinoma (PAa) were orthotopically implanted into nude mice, and the take rates were 74% for PG and 56% for PAa. Invasive tumors located in hilar region or peripheral part of lung, or spread diffusely, which were quite similar to three macroscopic types of primary lung carcinomas and different from well-circumscribed masses of ectopically implanted tumors. It is interesting to point out that the metastatic incidence of PAa cells in regional lymph nodes was 56% when implanted orthotopically which was even higher than the metastatic incidence in beige nude mice bearing subcutaneous tumors. Collectly, the results demonstrate that the orthotopic implantation of human lung carcinomas favour the expression of metastatic phenotype suitable for experimental study of metastasis. The comparation of expressions of oncogenes and antioncogenes in orthotopically implanted tumors with those in subcutaneously implanted tumors of the same kind will contribute to searching for molecular basis of tumor invasion and metastasis.