| 利用离体培养技术打破桂花种子休眠的试验 |
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| 引用本文： | 袁王俊,董美芳,尚富德.利用离体培养技术打破桂花种子休眠的试验[J].南京林业大学学报(自然科学版),2004,28(Z1):91-93. |
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| 作者姓名： | 袁王俊 董美芳 尚富德 |
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| 作者单位： | 河南大学生命科学院,河南,开封,475001 |
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| 基金项目： | 河南省自然科学基金资助项目(0311031400) |
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| 摘 要： | 桂花种子成熟后胚有后熟作用,胚乳中可能含有抑制胚萌发的物质,使用赤霉素和3个月的层积处理也不能促使其萌发。将桂花种子进行3个月层积处理后,进行离体培养,培养基中加入0.5 mol/L BA,1.0 mol/L BA 0.5NAA可以打破种子的休眠,促进萌发。
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| 关 键 词： | 桂花 种子休眠 胚 离体培养 |
| 文章编号： | 1000-2006(2004)增-0091-03 |
| 修稿时间： | 2004年5月30日 |
Preliminary Study on Breaking the Seed Dormancy of Osmanthus fragrans by in Vitro Culture |
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| YUAN Wang-jun,DONG Mei-fang,SHANG Fu-de.Preliminary Study on Breaking the Seed Dormancy of Osmanthus fragrans by in Vitro Culture[J].Journal of Nanjing Forestry University(Natural Sciences ),2004,28(Z1):91-93. |
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| Authors: | YUAN Wang-jun DONG Mei-fang SHANG Fu-de |
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| Abstract: | Osmanthus fragrans embryo has after-ripening when the seed is mature. There is perhaperps restraining substances in endosperm. The seed does not germinate even if it is dealed with GA or stored for three months in sand. By in vitro culture and adding 0.5 mol/L BA,1.0 mol/L BA 0.5 mol/L NAA to culture media, the seed dormancy can be broken after stored for three months in sand. |
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| Keywords: | Osmanthus fragrans Seed dormancy Embryo In vitro culture |
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