|
|||||
|
|
| 丁草胺对中华大蟾蜍蝌蚪的遗传毒性 | |
| 引用本文： | 林玲,姚丹,钟碧瑾,房振华,王楠楠,耿艳,耿宝荣.丁草胺对中华大蟾蜍蝌蚪的遗传毒性[J].福建师范大学学报(自然科学版),2009,25(6). |
| 作者姓名： | 林玲 姚丹 钟碧瑾 房振华 王楠楠 耿艳 耿宝荣 |
| 作者单位： | 1. 福建师范大学生命科学学院,福建,福州,350108 2. 沈阳市艺术幼儿师范学校,辽宁,沈阳,110015 3. 福建师范大学附属中学,福建,福州,350007 |
| 基金项目： | 福建省科技厅基金资助项目，福建省自然科学基金资助项目 |
| 摘 要： | 以中华大蟾蜍(Bufo gargarizans gargarizans)蝌蚪为研究对象,采用微核实验和碱性单细胞凝胶电泳法(又称彗星实验,SCGE)检测不同质量浓度(0.1,0.2,0.3,0.4 mg/L)的丁草胺溶液对中华大蟾蜍蝌蚪红细胞的遗传毒性.结果表明:在实验室条件下,丁草胺质量浓度越高,蝌蚪红细胞的微核率和核异常率越显著.经丁草胺溶液处理24 h,蝌蚪红细胞的细胞损伤率及DNA损伤程度(彗星DNA长宽比)出现极显著的提高,并与丁草胺质量浓度呈显著的线性关系.研究表明丁草胺对两栖动物具有遗传毒性作用,同时也说明微核实验和碱性单细胞凝胶电泳法是检测环境污染对两栖动物遗传毒性的合适方法. |
| 关 键 词： | 中华大蟾蜍蝌蚪 丁草胺 遗传毒性 微核实验 彗星实验 |
Genotoxicity of the Herbicide Butachlor on Bufo gargarizans gargarizans Tadpoles |
|
| LIN Ling,YAO Dan,ZHONG Bi-jin,FANG Zhen-hua,WANG Nan-nan,GENG Yan,GENG Bao-rong.Genotoxicity of the Herbicide Butachlor on Bufo gargarizans gargarizans Tadpoles[J].Journal of Fujian Teachers University(Natural Science),2009,25(6). | |
| Authors: | LIN Ling YAO Dan ZHONG Bi-jin FANG Zhen-hua WANG Nan-nan GENG Yan GENG Bao-rong |
| Institution: | LIN Ling1,YAO Dan2,ZHONG Bi-jin1,FANG Zhen-hua1,WANG Nan-nan1,GENG Yan3,GENG Bao-rong1*(1.College of Life Sciences,Fujian Normal University,Fuzhou 350108,China,2.Shenyang Art and Children Normal School,Shenyang 110015,3.Affiliated High School of Fujian Normal University,Fuzhou 350007,China) |
| Abstract: | Micro-nuclear test and the alkaline single-cell gel electrophoresis assay(SCGE) or comet assay was performed to examine the genotoxicity of butachlor onerythrocytes of Bufo gargarizans gargarizans tadpoles exposed to increasing concentrationof the tested substance(0.1,0.2,0.3,0.4 mg/L).The results showed that both micro-nuclear frequency and nuclear abnormity frequency were significantly increased with theincreasing concentration of butachlor in laboratory condition.Tadpoles treated for 24 h hadsignificant,... |
| Keywords: | Bufo gargarizans gargarizans tadpole butachlor genotoxicity micro-nuclear test comet assay |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |