| Transgenic tobacco plants harboring tomato proteinase inhibitor II gene and their insect resistance |
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| 引用本文： | XIE Xianzhi,HUANG Meijuan,WU Naihu. Transgenic tobacco plants harboring tomato proteinase inhibitor II gene and their insect resistance[J]. 自然科学进展(英文版), 2002, 12(3): 198-203 |
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| 作者姓名： | XIE Xianzhi HUANG Meijuan WU Naihu |
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| 作者单位： | Institute of Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China,College of Life Sciences, Peking University, Beijing 100871, China,Institute of Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China |
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| 基金项目： | Supported by the Langqidao Company, Fuzhou Municipality, Fujian Province |
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| 摘 要： | The plant expression vectors pBCT2 and pBT2 were constructed with the cDNA sequence (tin2) and genomic DNA sequence (tin2i) of tomato proteinase inhibitor II gene respectively. Then the two expression vectors were transferred into tobacco via the Agrobacterium tumefaciens strain LBA4404, and transgenic tobacco plants were generated. Molecular analysis and trypsin activity assay showed that both cDNA and genomic DNA were expressed properly in the transgenic plants. Insecticidal activities in these transgenic plants indicated that transgenic tobacco plants carrying tin2i sequence were more resistant to 2-instar larvae of Heliothis armigera Hubner than those carrying tin2 sequence. Therefore the intron of tin2i sequence might be a contributor to insecticidal activity of the transgenic tobacco.
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| 关 键 词： | tomato proteinase inhibitor II gene transgenic tobacco insect-resistance |
Transgenic tobacco plants harboring tomato proteinase inhibitor II gene and their insect resistance |
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| XIE Xianzhi,HUANG Meijuan,WU Naihu. Transgenic tobacco plants harboring tomato proteinase inhibitor II gene and their insect resistance[J]. Progress in Natural Science, 2002, 12(3): 198-203 |
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| Authors: | XIE Xianzhi HUANG Meijuan WU Naihu |
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| Affiliation: | 1. Institute of Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China
2. College of Life Sciences, Peking University, Beijing 100871, China |
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| Abstract: | The plant expression vectors pBCT2 and pBT2 were constructed with the cDNA sequence (tin2) and genomic DNA sequence (tin2i) of tomato proteinase inhibitor II gene respectively. Then the two expression vectors were transferred into tobacco via the Agrobacterium tumefaciens strain LBA4404, and transgenic tobacco plants were generated. Molecular analysis and trypsin activity assay showed that both cDNA and genomic DNA were expressed properly in the transgenic plants. Insecticidal activities in these transgenic plants indicated that transgenic tobacco plants carrying tin2i sequence were more resistant to 2-instar larvae of Heliothis armigera Hubner than those carrying tin2 sequence. Therefore the intron of tin2i sequence might be a contributor to insecticidal activity of the transgenic tobacco. |
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| Keywords: | tomato proteinase inhibitor II gene transgenic tobacco insect-resistance |
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