藻胆体稳定性的研究（Ⅴ）—pH对藻胆体稳定性的影响

不同ｐＨ对多变鱼腥藻（Ａｎａｂａｅｎａｖａｒｉａｂｉｌｉｓ）藻胆体荧光发射光谱，光能传递和解离的影响．在ｐＨ７磷酸缓冲溶液中，藻胆体处于稳定状态，不发生解离．在ｐＨ６，８，９，１０磷酸缓冲溶液中，藻胆体仍处于较稳定状态，没有解离．在ｐＨ５，５．５和１０．５磷酸缓冲溶液中，藻胆体发生弱解离．在ｐＨ４．５缓冲溶液中，藻胆体发生明显解离．在ｐＨ４和１１缓冲溶液中，藻胆体发生严重解离；藻红蓝蛋白，Ｃ－藻蓝蛋白和别藻蓝蛋白所捕获的光能已不能传递给末端发射体

The Effect of pH on the Stability of Phycobilisomes from Anabaena Variabilis

Room temperature and 77 K fluorescence,energy transfer and dissociation of phycobilisomes in different pH phosphate buffer were studied. Phycobilisomes in pH 7 buffer were stable,they were intact phycobilisomes.Phycobilisomes in pH 6,8,9,and 10 buffer weren't dissociated.Phycobilisomes in pH 5,5.5 and 10.5 buffer were dissociated weakly. Phycobilisomes in pH 4.5 buffer were dissociated evidently.When phycobilisomes in pH 4 and 11phosphate buffer,they were dissociated severely;energy traped by phycoerythrocyanin,C-phycocyanin and allophycocyanin couldn't transfer to terminal emitters.