| 野败型水稻的核质互作与基因差异表达 |
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| 引用本文： | 钱晓茵 高燕萍. 野败型水稻的核质互作与基因差异表达[J]. 复旦学报(自然科学版), 1998, 37(2): 163-167 |
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| 作者姓名： | 钱晓茵 高燕萍 |
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| 基金项目： | 国家科委攀登计划资助课题 |
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| 摘 要： | 运用差异展示方法对野败型细胞质雄性不育系珍汕９７Ａ及其相应的保持系珍汕９７Ｂ基因产物进行比较分析，发现这两个核基因组完全相同的品质间约有１０％的基因表达有差异；选取一个差异表达明显的ｃＤＮＡ成员Ｂ１３２进行Ｎｏｒｔｈｅｒｎ杂交发现，该基因在保持系中正常表达同源，在不育系中表达受到明显抑制，而在杂种一代中又正常代表达，在恢复系中表达水平也很低，这是一种典型的核质互作现象。
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| 关 键 词： | 细胞质雄性不育 核质互作 基因表达 野败型水稻 |
Interaction Between Nucleus and Cytoplasm and Gene Differential Expression in Rice CMS-WA Type |
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| Qian Xiaoyin, Gao Yanping, Yang Jinshui. Interaction Between Nucleus and Cytoplasm and Gene Differential Expression in Rice CMS-WA Type[J]. Journal of Fudan University(Natural Science), 1998, 37(2): 163-167 |
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| Authors: | Qian Xiaoyin Gao Yanping Yang Jinshui |
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| Affiliation: | Institute of Genetics |
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| Abstract: | About 10% of the whole genes express differently between rice CMS-WA line Zhenshan 97A and its conservative line Zhenshan 97B detected by DD-RT-PCR analysis. Furthermore, gene B132, gives a typical expression pattern of interaction between nucleus and cytoplasm. Compared to its expression in line B, gene B132 is highly inhibited in line A, while it shows common expression as Line B in F1, and its expression in restorative line is al- so as low as that in line A. B132 doesn't show RFLP within different lines in Southern hybridization patterns. |
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| Keywords: | DD-RT-PCR cytoplasmic male sterile interaction between nucleus and cytoplasm |
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