Co-localization of glucagon and pancreatic polypeptide in testudine pancreas |
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Authors: | W B Rhoten |
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Institution: | (1) Department of Anatomy and Cell Biology, UMDNJ-New Jersey Medical School, 07103 Newark, New Jersey, USA;(2) Cell Biology Unit, Medical School, University of Cape Town, Observatory 7925, Cape, (South Africa) |
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Abstract: | Summary Immunocytochemistry was carried out on sections of pancreas from the gopher tortoise,Gopherus polyphemus. Combined immunofluorescent and peroxidase-anti-peroxidase techniques showed unequivocally that some of the cells were immunoreactive for both glucagon and pancreatic polypeptide (PP). Antibodies directed against avian PP, bovine PP, and human PP all have a positive reaction. Co-localization of glucagon and PP in the pancreas of the gopher tortoise indicates that the occurrence of these hormones in the same cells is widespread in higher vertebrates.Acknowledgments. This work was supported in part by grants from the University of Cape Town, the M. Crossley bequest, the N. Atkinson bequest, and Herman and Caporn bequests. The generous gifts of antisera by Dr Chance and the late Dr Kimmel, and the gift of hormones by the Lilly Research Laboratories are gratefully acknowledged. The excellent technical assistance of J. Thompson and J. B. De Haan is also gratefully acknowledged. Please send all correspondence to the USA address. |
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Keywords: | Antibodies hormones pancreatic peptides localization immunocytochemical |
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