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盐胁迫对甜高粱幼苗抗氧化酶活性的影响
引用本文:姜慧,黄健,张云鹤,胡瑞芳,李玥莹. 盐胁迫对甜高粱幼苗抗氧化酶活性的影响[J]. 沈阳师范大学学报(自然科学版), 2012, 30(2): 289-292
作者姓名:姜慧  黄健  张云鹤  胡瑞芳  李玥莹
作者单位:沈阳师范大学化学与生命科学学院,沈阳,110034
基金项目:辽宁省自然科学基金资助项目(20092070);沈阳市科技局国际合作项目(1091241-6-00);辽宁“百千万人才工程”资助项目(2008921058)
摘    要:研究了不同浓度NaCl溶液(含量0、50、100、150 mmol/L)处理对4种甜高粱幼苗超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性以及脂质过氧化产物丙二醛含量的影响。结果表明,与对照相比(即NaCl浓度为0),50 mmol/L NaCl处理显著增加了SOD、POD和CAT活性,而100和150 mmol/L NaCl处理则抑制了3种抗氧化酶活性。NaCl胁迫导致了脂质过氧化发生,具体表现为随着NaCl处理的浓度增加,丙二醛也增加。4种供试甜高粱对NaCl胁迫的应答有差异,其中辽甜1号在所有供试浓度的NaCl胁迫下,3种抗氧化酶活性均高于其他3个品种,而丙二醛含量最低,表明辽甜1号对NaCl的耐受性是最强的。

关 键 词:甜高粱  NaCl  抗氧化酶  丙二醛

Effect of salt stress on antioxidative enzymes in sweet sorghum seedlings
JIANG Hui , HUANG Jian , ZHANG Yun-he , HU Rui-fang , LI Yue-ying. Effect of salt stress on antioxidative enzymes in sweet sorghum seedlings[J]. Journal of Shenyang Normal University(Natural Science Edition), 2012, 30(2): 289-292
Authors:JIANG Hui    HUANG Jian    ZHANG Yun-he    HU Rui-fang    LI Yue-ying
Affiliation:JIANG Hui,HUANG Jian,ZHANG Yun-he,HU Rui-fang,LI Yue-ying (College of Chemistry and Life Science,Shenyang Normal University,Shenyang 110034,China)
Abstract:This study investigated the effects of different concentrations of NaCl(0,50,100,and 150 mmol/L) on antioxidative enzymes activity including superoxide dismutase(SOD),peroxidase(POD) and catalase(CAT),and the content of malondialdehyde(MDA) in sweet sorghum seedlings.The results showed that the activities of SOD、POD and CAT were increased in plants exposed to 50 mmol/L NaCl compared to control,while the activities of these 3 enzymes were inhibited at 100 and 150 mmol/L NaCl.All tested concentrations of NaCl resulted in lipid peroxidation as indicated by MDA formation with a dose-effectiveness relationship.The NaCl sensibility was different among the four sweet sorghums,with the most tolerance occurring in No.1 of Liao Sweet,as shown by the highest antioxidative enzyme activity and the lowest MDA accumulation,which suggested that the No.1 of Liao Sweet may be a good candidate.
Keywords:sweet sorghum  NaCl  antioxidative enzymes  malondialdehyde
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