激素敏感脂酶(HSL)基因敲除小鼠认知行为的研究

目的:评估HSL基因敲除小鼠的学习记忆功能.方法:采用经典的Morris水迷宫实验,测试三种基因型小鼠的认知行为变化.结果:三种基因型小鼠的潜伏期几乎都逐渐缩短,雄性正常小鼠的潜伏期在1～2天,明显地短于HSL敲除小鼠(P<0.01);雌性正常小鼠的潜伏期在第3～5天,明显地短于HSL敲除小鼠(P<0.01),随后几天都无显著差异.第8天撤去平台,小鼠跨越原平台位置的次数无显著差异.结论:HSL敲除的小鼠都存在明显的认知水平功能障碍,雄性小鼠在前期差异显著,雌性小鼠在中期差异显著,但给予适当的学习训练后都能达到正常小鼠的认知水平.

Cognitive Behavioral Study in Hormone Sensitive Lipase (HSL) Knockout Mice

Objective： To see whether or not Hormone sensitive lipase （HSL） influence the learning and memory abilities in mice. Methods： three genotypes of mice were tested by Morris water maze. Results： The latency for finding the hidden platform in HSL knockout male mice showed significant longer than that in wild type mice at day 1 ~ 2 （ P 〈 0.01 ）. While in HSL knockout female,it was longer than that in control mice only at day 3 - 5 （P 〈 0. 01 ）. Times across the location of previous platform within all genotypes were indistinguishable at end of the test. Conclusion： HSL knockout mice exhibited lower ability in learning and memorizing at beginning of the test compared with wild type mice. There is a sex difference in knockout mice. However, this difference could be reduced gradually by proper training sessions.