A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity |
| |
Authors: | Lee Siu Sylvia Lee Raymond Y N Fraser Andrew G Kamath Ravi S Ahringer Julie Ruvkun Gary |
| |
Affiliation: | Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA. |
| |
Abstract: | We report a systematic RNA interference (RNAi) screen of 5,690 Caenorhabditis elegans genes for gene inactivations that increase lifespan. We found that genes important for mitochondrial function stand out as a principal group of genes affecting C. elegans lifespan. A classical genetic screen identified a mutation in the mitochondrial leucyl-tRNA synthetase gene (lrs-2) that impaired mitochondrial function and was associated with longer-lifespan. The long-lived worms with impaired mitochondria had lower ATP content and oxygen consumption, but differential responses to free-radical and other stresses. These data suggest that the longer lifespan of C. elegans with compromised mitochrondria cannot simply be assigned to lower free radical production and suggest a more complex coupling of metabolism and longevity. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|