人视网膜色素上皮细胞生长特性及可见光对其影响

 研究体外培养的人视网膜色素上皮细胞的生长特性及可见光对其影响.用倒置相差显微镜和透射电镜观察人视网膜色素上皮(hRPE)细胞形态和超微结构,用计数法和噻唑蓝(MTT)法绘制生长曲线.从光照时间、光照时有无血清、光照后是否换液、光照后培养时间这4方面考查可见光对hRPE细胞的损伤情况.实验结果表明,体外培养的hRPE细胞为多角形,有极性,细胞器正常,生长旺盛.(8423±359)lx的可见光光照2h就能对hRPE细胞造成显著损伤.光照时间为5h,光照时培养液中含10%胎牛血清,光照后不换培养液,光照后培养时间为24h,此条件下hRPE损伤率为58%.体外培养的hRPE细胞具有正常的形态结构和生长规律.可见光可对hRPE细胞造成损伤,并且损伤程度有时间积累效应.因此,在日常生活中应尽可能避免阳光直射,减少光照时间,以降低视网膜光损伤发生率,保护眼睛.

The Growth Characteristics of Human Retinal Pigment Epithelium Cells and the Influence of Visual Light on the Characteristics

The research object is to study the growth characteristics of human Retinal Pigment Epithelium (hRPE) cells, and to investigate the damage to them which is induced by visual light. Inverted phase contrast microscope and transmission electron microscope were used to observe the morphology and microstructures of hRPE cells, respectively. Growth curve was depicted by both cells counting and MTT methods. Exposure time, FBS is free or not, replacing medium or not, and incubation time post irradiation were selected as the factors to estimate the photo-damage in hRPE cells. The results show that the hRPE cells incubated in vitro are polygonal and polarized, normal organelle and vigorous growth are also observed. The illumination for two hours with an intensity of (8,423±359)lx had already caused photo-damage. Exposed for five hours with 10% FBS in medium which is not replaced post irradiation and incubated for 24h subsequently, the damage rate of hPRE cells goes up to 58%. In conclusion, the hRPE cells cultured in vitro show normal morphology and regular growth rhythm. Visual light could cause damage to hRPE cells and the damage degree is correlated with the illumination duration and post-incubating time. Therefore the illumination directly from the sun should be avoided and reducing the illumination duration in order to protect human eyes.