| 华北大黄愈伤组织培养及土大黄苷含量研究 |
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| 引用本文： | 王俊丽,陆远,刘坤,许东婷,刘名飞.华北大黄愈伤组织培养及土大黄苷含量研究[J].河北大学学报(自然科学版),2011,31(2):195-199. |
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| 作者姓名： | 王俊丽 陆远 刘坤 许东婷 刘名飞 |
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| 作者单位： | 1. 中央民族大学,生命与环境科学学院,北京,100081
2. 中央民族大学,生命与环境科学学院,北京,100081;河北经贸大学,生物科学与工程学院,河北,石家庄,050061 |
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| 基金项目： | "973"计划资助项目，植物化学与西部植物资源持续利用国家重点实验室开放课题资助项目，国家民委资助项目，"985"工程资助项目，"高等学校学科创新引智计划"资助项目，中央高校基本科研业务费专项资金资助项目 |
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| 摘 要： | 为有效保护和利用华北大黄野生资源,以野生华北大黄为材料,探讨了不同种类、不同质量浓度的植物生长调节剂对其愈伤组织诱导及增殖效应,并采用高效液相色谱对愈伤组织及野生植株中土大黄苷含量进行了分析.结果表明:MS培养基中单独添加不同质量浓度的6-BA,不利于愈伤组织的增殖;培养基中单独添加不同质量浓度的NAA和2,4-D,对...
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| 关 键 词： | 华北大黄 愈伤组织 土大黄苷 |
Callus Proliferation and Rhaponticin Accumulation of Rheum franzenbachii Munt. |
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| WANG Jun-li,LU Yuan,LIU Kun,XU Dong-ting,LIU Ming-fei.Callus Proliferation and Rhaponticin Accumulation of Rheum franzenbachii Munt.[J].Journal of Hebei University (Natural Science Edition),2011,31(2):195-199. |
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| Authors: | WANG Jun-li LU Yuan LIU Kun XU Dong-ting LIU Ming-fei |
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| Institution: | 1(1.College of Life and Environmental Sciences,Minzu University of China,Beijing 100081,China;2.College of Biology Science and Engineering,Hebei University of Economics and Business,Shijiazhuang 050061,China) |
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| Abstract: | The effects of different plant growth regulators on callus proliferation were discussed,using wild plant of Rheum franzenbachii as materials in this study,and contents of rhaponticin in samples of callus and wild plants was analyzed by HPLC.The results showed that the MS medium plus different mass concentration of 6-BA lonely was not conducive to the proliferation of callus,and different mass concentration of NAA and 2,4-D lonely played a role for the proliferation.6-BA in combination with lower mass concentration of NAA or 2,4-D was very suitable for callus proliferation.Rhaponticin were detected in three types of callus,and the mass fraction of two types of calluses was as high as 1.45%~1.54%,3.63~3.85 times compared to that of wild plants. |
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| Keywords: | Rheum franzenbachii callus rhaponticin |
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