| 泛素和PR1a信号肽融合基因植物表达载体的构建 |
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| 引用本文： | 史芳芳,周鹏,武冬梅,祝建波. 泛素和PR1a信号肽融合基因植物表达载体的构建[J]. 石河子大学学报(自然科学版), 2006, 24(3): 265-269 |
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| 作者姓名： | 史芳芳 周鹏 武冬梅 祝建波 |
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| 作者单位： | 石河子大学生命科学学院,新疆石河子,832003;中国热带农业科学院热带作物生物技术国家重点实验室,海南海口,571101;石河子大学绿洲生态生物技术中心,新疆石河子,832003 |
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| 摘 要： | 泛素（Ubiquitin）融合蛋白策略在近年来已被应用于在植物中提高外源蛋白的产量。信号肽（Signal Peptide）使外源蛋白定向运输到细胞内的特定部位。本研究是在植物高效表达载体pBin438的基础上，采用三引物PCR法（TP-PCR）技术，将拟南芥（Ambidopsis）泛素基因与烟草病程相关蛋白PR1a基因的信号肽序列体外重组；并将重组的融合基因定向克隆到植物表达载体pBLG中，获得了含有泛素和PR1a信号肽序列的植物表达载体pBLG-UP。这为进一步验证外源基因在植物体内的高效表达奠定了基础。
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| 关 键 词： | 泛素 PR1a信号肽 三引物PCR 融合基因 表达载体 |
| 文章编号： | 1007-7383（2006）03-0265-05 |
| 收稿时间： | 2006-03-10 |
| 修稿时间： | 2006-03-10 |
Construction of Plant Expression Vector of Fusion Gene Ubiquitin and Signal Peptide Sequence of Pathogenesis-related Protein 1a |
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| SHI Fang-fang,ZHOU Peng,WU Dong-mei,ZHU Jian-bo. Construction of Plant Expression Vector of Fusion Gene Ubiquitin and Signal Peptide Sequence of Pathogenesis-related Protein 1a[J]. Journal of Shihezi University(Natural Science), 2006, 24(3): 265-269 |
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| Authors: | SHI Fang-fang ZHOU Peng WU Dong-mei ZHU Jian-bo |
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| Abstract: | The fusion protein of ubiquitin had been used to enhance output of exterior protein.Signal peptide sequence can convey extraneous protein to a specifical position of cell.Ubiquitin and signal peptide sequence of pathogenesis-related protein 1a(PRS) were recombined together by TP-PCR in the research based on pBin438 of the efficient strongly plant expression vector.The fusion gene was constructed on expression vector pBLG(named pBLG-UP).The experiment would lay a foundation for accumulating protein effectively in the plant. |
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| Keywords: | ubiquitin pathogenesis-related protein la signal peptide TP-PCR fusion gene expression vector |
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