Purification of mouse liver thymidylate synthetase by affinity chromatography using 10-methyl-5,8-dideazafolate as the affinant |
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| Authors: | D. G. Priest M. T. Doig J. B. Hynes |
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| Affiliation: | (1) Departments of Biochemistry and Pharmaceutical Chemistry, Medical University of South Carolina, 171 Ashley Avenue, 29403 Charleston, South Carolina, USA |
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| Abstract: | Summary Thymidylate synthetase (methylenetetrahydrofolate: 2 -deoxyuridine-5-monophosphate C-methyltransferase; EC 2.1.1.45) from neonatal mouse liver has been purified 714-fold by affinity chromatography on aminohexylsepharose bound 10-methyl-5,8-dideazafolate.Supported by grant CA22754, awarded by the National Cancer Institute, DHEW. |
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