Comparison of intradermal pigeon crop-sac bioassay and double antibody radioimmunoassay for rat prolactin |
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| Authors: | M G Subramanian R P Reece |
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| Institution: | (1) Department of Animal Sciences, Cook College, Rutgers University, 08903 New Brunswick, New Jersey, USA;(2) Present address: Department of Gyn/Ob, Wayne State University School of Medicine, 275 E. Hancock, Avenue, 48201 Detroit, Michigan, USA |
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| Abstract: | Summary On an absolute basis, the intradermal pigeon crop-sac bioassay (PCA) gave results that were 20.5% higher than the radioimmunoassay (RIA) in rat anterior pituitary (AP) preparations. A highly significant correlation (r=0.87) was obtained between RIA (in  g) and PCA (in Reece-Turner units) when 58 medium samples obtained by culturing rat APs in vitro were assayed for prolactin (PRL) content.Supported by the Charles and Johanna Busch Memorial Fund and by Hatch Amended Funds, Rutgers University-The State University of New Jersey, New Brunswick, NJ 08903, USA.The authors acknowledge the generous supply of rat PRL reference preparation and iodination material for the RIA by NIAMDD, NIH. |
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