Adhesion of subsets of human blood mononuclear cells to porcine endothelial cells |
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Authors: | Xiaofeng Zhang Zhimin Feng Meifu Feng Hongfang Wang Ban Liqin |
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Institution: | (1) State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, 100080 Beijing, China |
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Abstract: | Cellular immune response is a major barrier to xenotransplantation, and cell adhesion is the first step in intercellular recognition.
Flow-cytometric adhesion assay has been used to investigate the differential adhesions of monocyte (Mo), natural killer cell
(NK) and T lymphocyte (T) present within human peripheral blood mononuclear cells (PBMC) to porcine aortic endothelial cells
(PAEC), and to demonstrate the effect of human interferon-γ (hIFN-γ) or/and tumor necrosis factor-α (hTNF-α) pretreatment
of PAEC on their adhesiveness for different PBMC subsets. The preferential sequence for PBMC subset binding to resting PAEC
is Mo, NK and T cells, among which T cells show the slightest adherence; hTNF-α can act across the species, and augment Mo,
NK and T cell adhesion ratios by 40%, 110% and 3 times, respectively. These results confirm at the cell level that host Mo
and NK cells are major participants in the cellular xenograft rejection, thereby, providing a prerequisite for further studying
the human Mo/NK-PAEC interactive mechanisms. |
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Keywords: | human peripheral blood mononuclear cell (PBMC) porcine aortic endothelial cell (PAEC) human tumor necrosis factor-α (hTNF-α ) cell adhesion phenotypic analysis |
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