| TAIL-PCR技术获取树状多节孢融合子的FSTs序列 |
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| 引用本文： | 迟彦,周东坡,平文祥,李姗姗,朱婧. TAIL-PCR技术获取树状多节孢融合子的FSTs序列[J]. 辽宁师范大学学报(自然科学版), 2007, 30(1): 96-99 |
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| 作者姓名： | 迟彦 周东坡 平文祥 李姗姗 朱婧 |
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| 作者单位： | 黑龙江大学,微生物重点实验室,黑龙江,哈尔滨,150080;大连大学,生物有机化学重点实验室,辽宁,大连,116622;黑龙江大学,微生物重点实验室,黑龙江,哈尔滨,150080 |
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| 基金项目： | 国家自然科学基金资助项目（30570025） |
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| 摘 要： | 采用TAIL-PCR技术,对随机选取的 6 个HDF-68阳性T-DNA转化子进行扩增,只利用1 d的时间,即获取了T-DNA标签侧翼序列（FSTs）,对其序列进行分析后,分析了影响TAIL-PCR扩增的主要因素.该项研究,为从T-DNA的突变子中分离紫杉醇产生菌产紫杉醇的相关基因提供了一条可行途径,也为阐明紫杉醇产生菌的代谢途径以及最终构建高产基因工程菌株奠定了基础.
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| 关 键 词： | TAIL-PCR FSTs 紫杉醇 |
| 文章编号： | 1000-1735（2007）01-0096-04 |
| 修稿时间： | 2006-01-18 |
Obtaining FSTs of Nodulisporium sylviforme fusant by TAIL-PCR techniques |
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| CHI Yan,ZHOU Dong-po,PING Wen-xiang,LI Shan-shan,ZHU Jing. Obtaining FSTs of Nodulisporium sylviforme fusant by TAIL-PCR techniques[J]. Journal of Liaoning Normal University(Natural Science Edition), 2007, 30(1): 96-99 |
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| Authors: | CHI Yan ZHOU Dong-po PING Wen-xiang LI Shan-shan ZHU Jing |
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| Affiliation: | 1. Key Laboratory of Microbe, Heilongjiang University, Harbin 150080,China; 2. Key Laboratory of Bio-organie Chemistry, Dalian University, Dalian 116622 ,China |
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| Abstract: | TAIL-PCR technique was performed to get the flanking sequence of T-DNA tags(FSTs) from six randomly selected HDF-68 positive T-DNA transformants only in one day time.After analysis of its sequences,the main factors of influencing TAIL-PCR amplification were discussed.This study provided a feasible way to seperate taxol biosynthesis related genes for taxol-producing fungi from T-DNA mutants,and also laid solid foundation for clarifying taxol pathway in taxol-producing fungi and for finally constructing high-yield gene engineering strain. |
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| Keywords: | TAIL-PCR FSTs Taxol |
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