| Pin1的细胞定位调控Rb蛋白的磷酸化状态 |
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| 引用本文： | 刘瑞红,谢刚,肖智雄.Pin1的细胞定位调控Rb蛋白的磷酸化状态[J].四川大学学报(自然科学版),2016,53(2):419-423. |
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| 作者姓名： | 刘瑞红 谢刚 肖智雄 |
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| 作者单位： | 四川大学生命科学学院生长代谢与衰老研究中心/生物资源和生态环境教育部重点实验室;四川省中西医结合医院;四川大学生命科学学院生长代谢与衰老研究中心/生物资源和生态环境教育部重点实验室;四川大学生命科学学院生长代谢与衰老研究中心/生物资源和生态环境教育部重点实验室 |
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| 基金项目： | 国家自然科学基金（31171362） |
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| 摘 要： | 为了研究Pin1和Rb蛋白的细胞定位、Pin1调控Rb磷酸化的相关机制,本研究构建了慢病毒载体pLVX-Flag-HA-Pin1和Plko.1-shRNA,包装病毒感染人非小细胞肺癌(H1299),免疫印迹检测Pin1蛋白表达水平,采用免疫荧光染色、免疫组化技术研究蛋白的定位.结果表明,沉默Pin1可降低Rb的磷酸化并抑制细胞的增殖;培养的肿瘤细胞和肿瘤组织中,Pin1可定位到细胞质中,而胞质定位的Pin1也可增加Rb的磷酸化.
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| 关 键 词： | Pin1 视网膜母细胞 瘤蛋白 细胞定位 磷酸化 |
| 收稿时间： | 2014/10/12 0:00:00 |
| 修稿时间： | 4/8/2015 12:00:00 AM |
Cellular localization of Pin1 regulates the phosphorylation status of Rb |
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| LIU Rui-Hong,XIE Gang and XIAO Zhi-Xiong.Cellular localization of Pin1 regulates the phosphorylation status of Rb[J].Journal of Sichuan University (Natural Science Edition),2016,53(2):419-423. |
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| Authors: | LIU Rui-Hong XIE Gang and XIAO Zhi-Xiong |
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| Institution: | Center of Growth, Metabolism and Aging, Key Laboratory of Biological Resources and Ecological Environment
of Ministry of Education, College of Life Sciences,Sichuan University;Sichuan Integrative Medicine Hospital;Center of Growth, Metabolism and Aging, Key Laboratory of Biological Resources and Ecological Environment
of Ministry of Education, College of Life Sciences,Sichuan University;Center of Growth, Metabolism and Aging, Key Laboratory of Biological Resources and Ecological Environment
of Ministry of Education, College of Life Sciences,Sichuan University |
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| Abstract: | In order to study the intracellular localization of Pin1 and Rb protein and the mechanisms of Pin1 regulated Rb phosphorylation, lentivirus vector pLVX Flag HA Pin1 and Plko.1 shRNA were constructed. After lentiviral packaging and infection, Pin1 protein level were detected by western blot in human non small cell 1ung carcinoma cell(H1299).Pin1 and Rb protein localization were studied by immunofluorescence staining and immunohistochemistry. The data demonstrated that knockdown of Pin1 result in a decrease of Rb phosphorylation and cellular viability. Pin1 can localize to cytoplasm in cultured tumor cells and tumor tissues, while cytoplasmic Pin1 can increase the phosphorylation of Rb. This is a novel mechanism for Pin1 in regulating Rb function. |
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| Keywords: | Pin1 Retinoblastoma protein cellular localization phosphorylation |
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