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| 锰氧化还原菌Exiguobacterium sp·MB4酶学特性研究 | |
| 引用本文： | 赵焱,李冬,曾涛涛,张杰真.锰氧化还原菌Exiguobacterium sp·MB4酶学特性研究[J].黑龙江大学自然科学学报,2009,26(5). |
| 作者姓名： | 赵焱 李冬 曾涛涛 张杰真 |
| 作者单位： | 1. 哈尔滨工业大学市政环境工程学院,哈尔滨,150090 2. 北京工业大学北京市水质科学与水环境恢复工程重点实验室,北京,100022 3. 哈尔滨工业大学市政环境工程学院,哈尔滨,150090;北京工业大学北京市水质科学与水环境恢复工程重点实验室,北京,100022 |
| 基金项目： | Supported by National Natural Science Foundation of China(50578001) |
| 摘 要： | 对锰氧化还原菌Exiguobacterium sp·MB4的酶学特性进行了研究。研究结果表明菌种Exig-uobacterium sp·MB4具有较强的胞外酶锰氧化还原活性。在20～25℃,pH中性偏碱条件下菌株均表现出较强的酶活力。最大反应速度为6.13mg·mL-1.min-1,米氏常数KM为155.8mg·mL-1。Fe2+、Al3+、Mg2+、Cu2+和Zn2+对锰氧化还原菌酶活力具有促进作用,Hg2+明显抑制菌株Exiguobacteriumsp.MB4的酶活力。 |
| 关 键 词： | 锰还原菌 酶活力 锰氧化酶 KM Vm |
Enzyme characteristics of manganese redox bacterium exiguobacterium sp·MB4 |
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| ZHAO Yan,LI Dong,ZENG Tao-tao,ZHANG Jie.Enzyme characteristics of manganese redox bacterium exiguobacterium sp·MB4[J].Journal of Natural Science of Heilongjiang University,2009,26(5). | |
| Authors: | ZHAO Yan LI Dong ZENG Tao-tao ZHANG Jie |
| Institution: | 1.School of Municipal and Environmental Engineering;Harbin Institute of Technology;Harbin 150090;China;2.Beijing Key Lab of Water Quality Science and Water Environment Recovery Engineering;Beijing University of Technology;Beijing 100022;China |
| Abstract: | Enzyme characteristics of manganese redox bacteria were investigated.The result showed that manganese redox bacteria,Exiguobacterium sp·MB4,has strong exoenzymes activity.The optimum temperture of enzyme activity was 20～25 ℃ and the optimum range of pH value was 7～8.The K_M and V_m were 155.8 mg·L~(-1) and 6.13 mg·L~(-1)·min~(-1),respectively.Fe~(2+),Al~(3+),Cu~(2+),Zn~(2+) and Mg~(2+) take as a catalytic role on manganese oxidoreductase,while Hg~(2+) inhibiting enzyme activity significantly. |
| Keywords: | manganese redox bacteria enzyme activity manganese oxidoreductase KM Vm |
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