恒定磁场对神经元延迟整流钾通道特性的影响

为了研究中等强度恒定磁场（静磁场）对神经细胞离子通道特性的影响，利用线圈及磁铁产生3mT、30mT和100mT的恒定磁场，作用于急性分离的小鼠额叶皮层锥体神经元，应用全细胞膜片钳技术研究其延迟整流钾通道特性．实验发现，中等强度恒定磁场使神经细胞延迟整流钾通道电流峰值减小．磁场作用可显著改变激活过程，使激活曲线的半数激活电压和斜率因子发生改变．结果表明，3mT、30mT和100mT恒定磁场可改变神经元延迟整流钾通道特性，从而影响神经元的生理功能．

Effects of Static Magnetic Fields on Characteristics of Neuron Delayed Rectifier Potassium Channel

To study the impact of moderate intensity static magnetic field on the characteristics of delayed rectifier potassium channel, acutely isolated mouse prefrontal cortex pyramidal neurons were exposed to 3 mT, 30 mT and 100 mT static magnetic fields,respectively,generated by a coil and a magnet, and the whole-cell patch clamp technique was applied. The experiment results reveal that static magnetic field reduces the amplitudes of IK and the static magnetic field exposure changes the activation process of IK significantly. The half-activation voltage and the slope factor of the activation curves change due to the magnetic field exposure. These results show that static magnetic fields of 3 mT, 30 mT and 100 mT can change the characteristics of delayed rectifier potassium channel, and physiological functions of neurons are adjusted as the results of static magnetic fields exposure.