Separation of canavanine and canaline by high performance liquid chromatography |
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| Authors: | J N van Balgooy |
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| Institution: | Department of Neurobiochemistry, Beckman Research Institute of the City of Hope, Duarte, California 91010. |
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| Abstract: | The plant amino acid canavanine and its hydrolytic product canaline were successfully separated and identified by Reverse Phase High Performance Liquid Chromatograph (RP-HPLC). This procedure was used to demonstrate the arginase-mediated cleavage of canavanine to yield canaline and urea, and the subsequent formation of a Schiff's base complex between canaline and pyridoxal phosphate. Both aforementioned reactions were demonstrated by RP-HPLC. |
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