野生树鼩可培养细菌和真菌携带情况的调查

目的对树鼩携带的可培养微生物群系进行初步调研,为制定树鼩的微生物学等级标准提供依据。方法采集61只野生树鼩呼吸道分泌物、肠道内容物和体表毛发,接种于多种培养基进行细菌分离培养,并进行生化、药敏和16SrRNA测序鉴定。结果在所采集的样品中共分离和检测到可培养的16个科32个属56种细菌及10个属的11种真菌;以肠杆菌属、葡萄球菌属、链球菌属和支原体属的携带率最高。其中常见病原菌有甲型副伤寒沙门氏菌、金黄色葡萄球菌、嗜肺巴斯德杆菌、肺炎链球菌、空肠弯曲菌、弗劳地柠檬酸杆菌、鲍氏志贺菌和支原体等。结论树鼩携带细菌和真菌的属种丰富、复杂,存在着多种人兽共患疫病的病原微生物。

The Survey of Culturable Bacteria and Fungi in Wild Tree Shrews

Objective To providet the basis for the development of national standards of experimental animals of tree shrew by means of preliminarily investigating the culturable microbiome.Method Collection of respiratory secretions,intestinal contents and hair samples of 61 wild tree shrews,cultured and isolated in a variety of corresponding medium and identified the isolated strains and DNA samples by biochemical isolates,susceptibility and 16SrRNA sequencing.Result We isolated and detected the 56 species of 32 genus of 16 family bacteria and the 11 species of 10 genus fungi of the genus.In the isolated,the carrier rate of Enterobacter,Staphylococcus,Streptococcus and Mycoplasma were the highest.There were the common pathogens,Salmonella paratyphi A,Staphylococcus aureus,Pasteurella pneumotropica,Streptococcus pneumoniae,Campylobacter jejuni,Citrobacter freundii,Shigella boydii and Mycoplasma sp..Conclusion The tree shrew microbiome is abundant and complex,and there are a variety of zoonotic bacterial pathogens.