多金属氧酸盐的抗艾滋病毒活性和机理的探讨

采用MTT方法和HIV P24抗原ELISA检测方法,在人的T淋巴细胞(MT-4)内,对24种多金属氧酸盐进行了抗艾滋病毒活性和毒性测定.结果显示,具有Keggin结构的还原型多金属氧酸盐(杂多蓝)是艾滋病毒的理想抑制剂,在非毒性剂量范围内显示出优于母体的抗艾滋病毒活性,其中最突出的是含有甘氨酸的Keggin型钨锗酸四电子杂多蓝(HPBG-110).研究表明,在人的淋巴细胞H9和人的外周边血液细胞(PBMC)内,HPBG-110对艾滋病毒标准株及临床分离毒株有较强的抑制活性,连续给药12代没有出现明显的耐药性.抑制合胞体的形成及干扰病毒向靶细胞的吸附可能是导致这类化合物抗艾滋病毒活性的原因.

Groping after anti-HIV activity and mechanism of polyoxometalates

The anti-HIV activities and toxicities of more than twenty polyoxometalates have been determined in human T lymph cells(MT-4)by MTT method and evaluated by ELISA of HIV-P24 antigen.Sixteen Keggin structure reductive polyoxometalates (heteropoly blues)are perfect inhibitors to HIV and they show anti-HIV activity superior to their parents in non-toxicity dosage range.Among them the most outstanding is glycin-containing Keggin type four-electron polyoxotungstogermanate heteropoly blue (HPBG-110).An in depth research shows that HPBA-110 has strong inhibitory activity to HIV standard strains and clinical separated strains in human lymph cells H9 and PBMC.Obvious drug resistance didn't appear when drug was supplied successively for 12 generations.The following mechanisms might be considered for their anti-HIV activity,namely,(1)inhibition of syncytium formation,(2)disturbing the adsorption of HIV-1 virus to target cells.