Chromate reduction inStreptomyces |
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Authors: | S. Das A. L. Chandra |
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Affiliation: | (1) Department of Microbiology, Bose Institute, 700 009 Calcutta, (India) |
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Abstract: | Summary Streptomyces species 3M grew in peptone yeast extract medium with 1000 g/ml K2Cr2O7. Incubation of the chromate with different cell fractions in the presence of NADH and NADPH resulted in a decrease of Cr6+ in the reaction mixture. The level of Cr6+ was reduced by 82.7% by a particulate cell fraction obtained by centrifugation at 105,000×g for 1 h, in the presence of NADH. The reducing enzyme was associated with this cell fraction. The enzyme was constitutive and reduced Cr6+ to Cr3+. |
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Keywords: | Streptomyces chromate tolerance reducing enzyme reduction of Cr6+ to Cr3+ |
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