负载纳米银的聚氨酯胶束的抗菌性及细胞毒性的研究

本文研究了三类聚乙二醇接枝位置不同且表面负载纳米银的聚氨酯胶束的抗菌性能及生物相容性。通过DLS表征了聚氨酯在负载纳米银后的粒径和电位变化,紫外分光光度法检测聚氨酯负载的纳米银的含量。MIC表征了三类聚合物的抗菌性能,细胞活性实验和溶血实验表征了其复合材料的生物相容性。复合材料的内核PCL在脂肪酶作用下降解释放小尺寸纳米银从而提高抗菌性。实验数据表明负载纳米银的PEG-g-PU胶束的抗菌性能和生物相容性都优于其他两种结构的胶束(PEG-c-PU、PEG-b-PU)。并针对负载纳米银的PEG-g-PU胶束研究其对成熟生物膜的影响。

Silver nanoparticles decorated polyurethane micelles showing good bactericidal activities and low cytotoxicity to HUVEC and erythrocytes

This paper studies the antibacterial properties and biocompatibility of silver nanoparticles decorated polyurethane micelles with different grafting positions of polyethylene glycol were studied. DLS was used to characterize the particle size and potential change of silver nanoparticles decorated polyurethane micelles, and the content of nano silver was measured by UV spectrophotometry. The antibacterial properties of three kinds of polymers were characterized by MIC, and the biocompatibility of the composites was characterized by Cell viability assay and hemolysis assay. Moreover, at the presence of lipase, the polymer matrix of nanocomposites is subject to degradation and the small silver nanoparticles were released. The experimental data show that the antibacterial properties and biocompatibility of silver nanoparticles decorated PEG-g-PU micelles are superior to those of the other two kinds of micelles (PEG-c-PU, PEG-b-PU). The effect of silver nanoparticles decorated PEG-g-PU micelles on the mature biofilm was studied.