| 祛痰止咳颗粒指纹图谱研究 |
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| 引用本文： | 郑文燕,王晓东,彭维,沈艺,苏薇薇.祛痰止咳颗粒指纹图谱研究[J].中山大学学报(自然科学版),2011,50(3). |
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| 作者姓名： | 郑文燕 王晓东 彭维 沈艺 苏薇薇 |
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| 作者单位： | 1. 中山大学生命科学学院,广东广州,510275
2. 广州市花城制药厂,广东广州,510555 |
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| 基金项目： | 广东省科技计划资助项目 |
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| 摘 要： | 采用HPLC法构建了祛痰止咳颗粒指纹图谱，色谱条件为Elite Hypersil BDS（4.6 mm×250 mm，5 μm）色谱柱，以甲醇(A)-0.15%(〖WTBX〗φ〖WTBZ〗)冰醋酸溶液(B)为流动相，线性梯度洗脱A（15%→75%），流速1.0 mL/min，柱温25 ℃，检测波长260 nm。该指纹图谱中，确定了19个共有峰，并采用对照品对照法及HPLC-ESI-MS/MS联用技术鉴定出10个黄酮类化合物。构建的指纹图谱，操作简便、准确可靠、重复性较好，为祛痰止咳颗粒质量控制提供了科学依据。
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| 关 键 词： | 祛痰止咳颗粒 高效液相色谱法 指纹图谱 |
| 收稿时间： | 2010-08-05; |
HPLC Fingerprint of Qutanzhike Granule |
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| ZHENG Wenyan,WANG Xiaodong,PENG Wei,SHEN Yi,SU Weiwei.HPLC Fingerprint of Qutanzhike Granule[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2011,50(3). |
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| Authors: | ZHENG Wenyan WANG Xiaodong PENG Wei SHEN Yi SU Weiwei |
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| Institution: | ZHENG Wenyan1,WANG Xiaodong2,PENG Wei1,SHEN Yi2,SU Weiwei 1 (1.School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China,2.Guangzhou Huacheng Pharmaceutical Company Limited,Guangzhou 510515,China) |
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| Abstract: | HPLC fingerprint of Qutanzhike Granule was established with Elite Hypersil BDS column（4.6 mm×250 mm,5 μm）,methanol(A) and 0.15% acetic acid(B) as mobile phase.The elution profile was: 0~95 min 15% to 75% A in B(linear gradient). The column temperature was set at 25 ℃ and the flow rate was 1.0 mL/min. The detective wavelength was at 260 nm. 19 common peaks were selected as the fingerprint peaks from 12 batches of the production. In the established fingerprint, 10 flavonoids were identified by the HPLC-ESI/MS/MS based on MS spetra and standard reference. The method can be used for quality control of Qutanzhike Granule with great precision,accuracy and good reproducibility. |
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| Keywords: | Qutanzhike Granule HPLC fingerprint |
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