| Isolation, characterization and functional analysis of a cdc48 homologue from tobacco BY-2 cells |
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| 作者姓名： | CHEN Zhiling YU Yi LIU Lina and XIA Guixian |
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| 作者单位： | College of Life Science，Capital Normal University，Beijing 100037，China; Institute of Microbiology，Chinese Academy of Sciences，Beijing 100080，China |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金 |
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| 摘 要： | The fission yeast Schizosaccharomyces pombe was used to identify genes from tobacco BY-2 cells that may play roles in cell cycle regulation. A cDNA encoding a protein homologous to the yeast CDC48 was isolated and the gene was designated as NtCDC48 . The cDNA contains an open reading frame coding for a predicted protein of 808 amino acids which comprises of two typical ATPase modules (aa 245-374 and aa 518-646) . Overexpression of NtCDC48 in tobacco BY-2 cells led to an increase in the mitotic index as well as to the formation of diffused mitotic spindles. NtCDC48-GFP fusion proteins are distributed ubiquitously through Gl to M phases, yet their subcellular localization varied regularly along with the cell cycle progression. These results indicate that NtCDC48 may play an important role in the regulation of cell cycle in BY-2 cells.
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Isolation, characterization and functional analysis of a cdc48 homologue from tobacco BY-2 cells |
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| CHEN Zhiling,YU Yi,LIU Lina and XIA Guixian.Isolation, characterization and functional analysis of a cdc48 homologue from tobacco BY-2 cells[J].Progress in Natural Science,2007,17(2):156-162. |
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| Authors: | CHEN Zhiling YU Yi LIU Lina XIA Guixian |
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| Abstract: | The fission yeast Schizosaccharomyces pombe was used to identify genes from tobacco BY-2 cells that may play roles in cell cycle regulation. A cDNA encoding a protein homologous to the yeast CDC48 was isolated and the gene was designated as NtCDC48 . The cDNA contains an open reading frame coding for a predicted protein of 808 amino acids which comprises of two typical ATPase modules (aa 245-374 and aa 518-646) . Overexpression of NtCDC48 in tobacco BY-2 cells led to an increase in the mitotic index as well as to the formation of diffused mitotic spindles. NtCDC48-GFP fusion proteins are distributed ubiquitously through Gl to M phases, yet their subcellular localization varied regularly along with the cell cycle progression. These results indicate that NtCDC48 may play an important role in the regulation of cell cycle in BY-2 cells. |
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| Keywords: | tobacco BY-2 suspension cells NtCDC48 spindle cell cycle |
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