| 高效液相色谱法测定葡萄酒中人工合成色素 |
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| 引用本文： | 宗万里,刘新才. 高效液相色谱法测定葡萄酒中人工合成色素[J]. 北京工商大学学报(自然科学版), 2013, 31(2): 58-61 |
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| 作者姓名： | 宗万里 刘新才 |
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| 作者单位： | 威海市产品质量监督检验所,山东威海,264209 |
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| 摘 要： | 建立了葡萄酒中柠檬黄、苋菜红、胭脂红、日落黄含量的高效液相色谱法测定方法.采用ODS-Cl8色谱柱,甲醇和0.02 mol/L乙酸铵的水溶液为流动相进行梯度洗脱,流速为0.8 mL/min,采用紫外检测器,检测波长为470 nm.该方法测定结果的相对标准偏差小于3%(n=6),平均回收率大于95%.
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| 关 键 词： | 高效液相色谱法 葡萄酒 柠檬黄 苋菜红 胭脂红 日落黄 |
Determination of Synthetic Food Pigments in Port Wine by HPLC |
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| ZONG Wan-li and LIU Xin-cai. Determination of Synthetic Food Pigments in Port Wine by HPLC[J]. Journal of Beijing Technology and Business University:Natural Science Edition, 2013, 31(2): 58-61 |
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| Authors: | ZONG Wan-li and LIU Xin-cai |
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| Affiliation: | Weihai Supervision and Inspection Institute of Product Quality;Weihai Supervision and Inspection Institute of Product Quality |
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| Abstract: | A method for the determination of tartrazine,amaranth,ponceau,and sunset yellow in wines by high performance liquid chromatography(HPLC)was established.The separation was achieved on a ODS-C18 column by a gradient elution with methanol and 0.02 mol/L aqueous ammonium acetate as mobile phase.The flow speed of the mobile phase was 0.8 mL/min and the determination was conducted at 470 nm through a UV detector.The average recovery of the method of real samples was more than 95%,and the RSD was less than 3%.Therefore,the method is suitable for the experiment. |
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| Keywords: | HPLC wine tartrazine amaranth ponceau sunset yellow |
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