| 酶电极法测定谷氨酸片中谷氨酸含量 |
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| 引用本文： | 孙士青,史建国,朱思荣,周万里,王少杰.酶电极法测定谷氨酸片中谷氨酸含量[J].山东科学,2007,20(4):33-36. |
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| 作者姓名： | 孙士青 史建国 朱思荣 周万里 王少杰 |
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| 作者单位： | 1. 山东省科学院中日友好生物技术研究中心,山东,济南,250014
2. 山东省科学院生物研究所,山东,济南,250014 |
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| 摘 要： | 以谷氨酸氧化酶(EC 1.4.3.11)与过氧化氢电极构成电流型酶电极,研究了谷氨酸酶电极分析法的各种分析条件及参数,并与旋光法对比测定谷氨酸片中谷氨酸含量。结果显示酶电极法测定结果与旋光法测定结果之间有较好的一致性,采用酶电极法测定谷氨酸片中谷氨酸含量具有专一性高、成本低、分析速度快等优点。
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| 关 键 词： | 谷氨酸氧化酶 酶电极 谷氨酸片 谷氨酸 |
| 文章编号： | 1002-4026（2007）04-0033-04 |
| 收稿时间： | 2007-04-25 |
| 修稿时间： | 2007年4月25日 |
Enzyme Electrode for the Determination of Glutamic Acid in Glutamic Acid Tablets |
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| SUN Shi-qing,SHI Jian-guo,ZHU Si-rong,ZHOU Wan-li,WANG Shao-jie.Enzyme Electrode for the Determination of Glutamic Acid in Glutamic Acid Tablets[J].Shandong Science,2007,20(4):33-36. |
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| Authors: | SUN Shi-qing SHI Jian-guo ZHU Si-rong ZHOU Wan-li WANG Shao-jie |
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| Institution: | 1. Centre of Biology, Shandong Academy of Sciences, Jinan 250014, China ; 2. Institute of Biology, Shandong Academy of Scienes , Jinan 250014, China |
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| Abstract: | To determine the concentration of glutamic acid in its tablets,glutamate oxidase was immobilized at a hydrogen peroxide electrode and the enzyme electrode was used for the amperometric determination of glutamic acid in glutamic acid tablets.The results show that the correlation coefficient between enzyme electrode and rotation is 0.9989,and that the concentration glutamic acid in its tablets can be determined specially,rapidly and inexpensively with enzyme electrode. |
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| Keywords: | glutamate oxidase enzyme electrode glutamic acid tablets glutamic acid |
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