链霉素诱导的2型糖尿病大鼠模型的肾脏功能变化

目的通过观察链霉素诱导的2型糖尿病大鼠模型肾脏功能的变化,以探讨此种模型是否适用于糖尿病肾损害的研究。方法采用链脲佐菌素(streptozotocin,STZ)辅以高脂饮食建立2型糖尿病大鼠模型,筛选造模成功的大鼠为糖尿病组,并与正常大鼠比较,造模后8周观察两组大鼠血糖、血尿素氮(blood urea nitrogen,BUN)、血肌酐(creatinine,CR)、血糖基化终末产物(Advanced glycation end products,AGE)、尿微量白蛋白、肾重指数的变化以及AGE受体(Receptor of AGE,RAGE)、蛋白激酶C(protein kinase C,PKC)、蛋白激酶A(protein kinase A,PKA)在肾脏的表达变化。结果糖尿病组的血糖、血尿素氮、血肌酐、血AGE、尿微量白蛋白以及肾重指数均明显高于正常组(P0.05),RAGE、PKC的mRNA以及蛋白表达水平均较正常组高(P0.05),PKA的mRNA和蛋白表达水平则较正常组低(P0.05)。结论 STZ诱导的2型糖尿病大鼠模型在造模后第8周肾脏功能存在明显的损害,并且导致肾脏损害的上游重要介质RAGE、PKC、PKA在第8周都有着明显的变化。此种模型能够用于糖尿病肾损害的研究,并且是观察中早期糖尿病肾病的较好的模型。

Changes of Renal Function in Type 2 Diabetic Rats Model Induced by Streptozotocin

Objective Observe the changes of renal function in the type 2 diabetic rats model induced by streptozotocin,and explore whether this model is suitable for the study of diabetes renal damage.Methods Diabetes mellitus animal models were induced by intraperitoneal injection of streptozotocin （STZ）and a high-fat diet.The diabetic rats are categorized as the diabetes group（DG,n =10）,the normal SD rats are categorized as the normal control group（NCG,n =8）.The blood glu-cose,blood urea nitrogen,serum creatinine,serum AGE,urine microalbumin and kidney weight index were measured 8 weeks after STZ injection in two groups.The expressions of RAGE,PKC and PKA in renal tissue were measured by Real Time PCR（RT -PCR）and Western blot.Results The blood glucose,blood urea nitrogen,serum creatinine,serum AGE,u-rine microalbumin and Kidney weight index level of DG group were significantly higher than NCG group（P 〈0.05）.The mRNA and protein expression of RAGE and PKC of DG group were higher than NCG group（P 〈0.05）.The mRNA and protein expression of PKA of DG group were significantly lower than NCG group.（P 〈0.05）.Conclusion The type 2 dia-betic rat model induced by STZ has significant damage of renal function in 8 weeks after STZ injection;The important up-stream factors of kidney damage-RAGE,PKC and PKA were significantly changed,Therefore this animal model could be used for the study of diabetic renal damage and it＆#39;s suitable for observation of early diabetic nephropathy.