酵母菌中海藻糖的提取方法与糖代谢研究

采用多种方法（三氯乙酸提取法、冷乙醇提取法、热乙醇提取法和热蒸馏水提取法）从酵母菌中提取非还原性二糖海藻糖，产率分别为１３．３８％，９．６８％，９．７５％和９．５５％．并对酵母菌中海藻糖的代谢进行研究，发现随培养条件恶化，酵母菌体内海藻糖含量有不同程度的提高．提高培养基中Ｔｒｉｓ含量，则海藻糖含量趋于下降

Extraction of Trehalose from Yeast Cells and Metablism of Trehalose in Yeast

The present paper introduces some methods for extracting non reducing bisaccharidetrehalose from yeast cells by which when 0.5 mol/L trichloacetic acid, cold ethanol, hot ethanol and hot distilled water were respectively used, the yields were 13.38%, 9.68%, 9.75% and 9.55%. The metabolism characters of trehalose in yeast has also been studied. The concentration of trehalose in yeast cells increased after a long time of incubation; and the higher concentration of trehalose was found also when yeast cells were cultured in a low nutrition medium, at higher tempratures and in the medium contained high concentration of salt; but the amount of trehalose in yeast was reduced at the high concentration of Tris in the medium.