| A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01) |
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| 作者姓名： | Lü Fushuang Lü Hong |
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| 作者单位： | QIN E抎e，ZHU Qingyu*，YU Man，FAN Baochang，CHANG Guohui，SI Bingyin，YANG Bao抋n，PENG Wenming，JIANG Tao，LIU Bohua，DENG Yongqiang，LIU Hong，ZHANG Yu，WANG Cui抏，LI Yuquan，GAN Yonghua，LI Xiaoyu，L Fushuang，TAN Gang，CAO Wuchun，YANG Ruifu Institute of Microbiology and Epidemiology，Chinese Academy of Military Medical Sciences，Beijing 100071，China WANG Jian，LI Wei，XU Zuyuan，LI Yan，WU Qingfa，LIN Wei，CHEN Weijun，TANG Lin，DENG Yajun，HAN Yujun，LI Changfeng，LEI Meng，LI Guoqing，LI Wenjie，L Hong，SHI Jianping，TONG Zongzhong，ZHANG Feng，LI Songgang，LIU Bin，LIU Siqi，DONG Wei，WANG Jun，Gane K-S Wong，YU Jun & YANG Huanming* Beijing Genomics Institute，Chinese Academy of Sciences，Beijing 101300; National Center for Genome Information，Beijing 101300，China |
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| 基金项目： | 科技部资助项目，国家自然科学基金 |
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| 摘 要： | Severe Acute Respiratory Syndrome (SARS) is a newly identified infectious disease1—5]. The global outbreak of SARS has been threatening the health of people worldwide and has killed 353 people and infected more than 5462 in 27 countries, as reported by WHO on April 29, 2003 (http://www.who.int/csr/sarscountry/en). Although it has been recognized that a variant of virus from the family of coronavirus might be the candidate pathogen of SARS1—5], its identity as the unique pathogen sti…
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A complete sequence and comparative analysis of a SARS-associated virus (Isolate BJ01) |
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| QIN E''de,ZHU Qingyu,YU Man,FAN Baochang,CHANG Guohui,SI Bingyin,YANG Bao''an,PENG Wenming,JIANG Tao,LIU Bohua,DENG Yongqiang,LIU Hong,ZHANG Yu,WANG Cui''e,Li Yuquan,GAN Yonghua,LI Xiaoyu,TAN Gang,CAO Wuchun,YANG Ruifu,WANG Jian,LI Wei,XU Zuyuan,LI Yan,WU Qingfa,LIN Wei,Chen Weijun,TANG Lin,DENG Yajun,HAN Yujun,LI Changfeng,LEI Meng,LI Guoqing,LI Wenjie,SHI Jianping,TONG Zongzhong,ZHANG Feng,Li Songgang,LIU Bin,LIU Siqi,DONG Wei,WANG Jun,Gane K-S Wong,YU Jun,YANG Huanming.A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)[J].Chinese Science Bulletin,2003,48(10). |
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| Authors: | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao'an PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui'e Li Yuquan GAN Yonghua LI Xiaoyu TAN Gang CAO Wuchun YANG Ruifu WANG Jian LI Wei XU Zuyuan LI Yan WU Qingfa LIN Wei Chen Weijun TANG Lin DENG Yajun HAN Yujun LI Changfeng LEI Meng LI Guoqing LI Wenjie SHI Jianping TONG Zongzhong ZHANG Feng Li Songgang LIU Bin LIU Siqi DONG Wei WANG Jun Gane K-S Wong YU Jun YANG Huanming |
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| Abstract: | The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). |
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| Keywords: | Severe Acute Respiratory Syndrome (SARS) coronavirus genome phylogeny |
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