| 紫花苜蓿耐盐突变体筛选 |
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| 引用本文： | 杨宝灵,姜健,封德全,邹福海,洪杰,杨军.紫花苜蓿耐盐突变体筛选[J].大连民族学院学报,2006,8(3):40-43. |
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| 作者姓名： | 杨宝灵 姜健 封德全 邹福海 洪杰 杨军 |
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| 作者单位： | 1. 大连民族学院,生命科学学院,辽宁,大连,116605
2. 大连市林业局,辽宁,大连,116023 |
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| 基金项目： | 辽宁省大连市科技局资助项目 |
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| 摘 要： | 以MS为基本培养基,研究不同激素对紫花苜蓿2号愈伤组织的诱导效果,筛选出最佳诱导愈伤组织培养基;以茎尖、茎段及叶片作为外植体,确定了叶片作为紫花苜蓿组织培养的最佳外植体;在1/4、1/3及2/3 3种不同海水培养基上分别诱导出紫花苜蓿耐盐愈伤组织,继代培养后得到不同耐盐程度的新株系,移栽后得到完整植株.
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| 关 键 词： | 紫花苜蓿 耐盐突变体 愈伤组织 筛选 |
| 文章编号： | 1009-315X（2006）03-0040-04 |
| 收稿时间： | 2005-10-12 |
| 修稿时间： | 2005年10月12 |
Screening of Alfalfa Mutant with Salt- tolerant Ability |
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| YANG Bao-ling JIANG-Jian FENG De-quan ZOU Fu-hai ZHANG Hong-yan.Screening of Alfalfa Mutant with Salt- tolerant Ability[J].Journal of Dalian Nationalities University,2006,8(3):40-43. |
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| Authors: | YANG Bao-ling JIANG-Jian FENG De-quan ZOU Fu-hai ZHANG Hong-yan |
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| Institution: | 1. College of Life Science, Dalian Nationalities University, Dalian Liaoning 116605, China; 2. Dalian Forestry Bureau, Dalian Liaoning 116023, China |
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| Abstract: | The effects of different plant hormones on the alfalfa callus induction were investigated with MS as the basic culture medium.Consequently,the best culture medium for callus induction was selected out.Compared with stem tine and stem section,lamina was the best explant for alfalfa tissue culture.The callus of alfalfa which was induced from the medium containing 1/4,1/3,or 2/3 of natural seawater was successfully regenerated and transferred into the soil. |
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| Keywords: | Alfalfa Mutant with Salt-tolerant Callus Screening |
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