| 绞股蓝热处理产物对A549细胞的抑制活性 |
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| 引用本文： | 朴香兰,吴倩,杨静,刘慧敏. 绞股蓝热处理产物对A549细胞的抑制活性[J]. 中央民族大学学报(自然科学版), 2012, 21(1): 49-53 |
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| 作者姓名： | 朴香兰 吴倩 杨静 刘慧敏 |
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| 作者单位： | 中央民族大学中国少数民族传统医学研究院,北京,100081 |
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| 基金项目： | 国家自然科学基金项目(No.30973960;81011140347;81181240306);国家民委科研项目(No.09ZY17) |
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| 摘 要： | 本文比较了绞股蓝(Gynostemma pentaphyllum)在热处理前后的甲醇提取物对肺癌A549细胞的抑制活性.采用绞股蓝在温度125oC、压力0.24MPa条件下,加热处理3h,用12倍量的80%甲醇超声提取1h,利用液质联用分析方法,分析了绞股蓝热处理前后的甲醇提取物中色谱峰的变化,并对它们进行肺癌A549细胞的抑制活性检测.结果发现热处理使绞股蓝中的成分有转化,而且绞股蓝的热处理产物对肺癌A549细胞的抑制活性显著增强.由此得出,热处理可产生绞股蓝成分的转化,而且对肺癌A549细胞的抑制活性增强,为绞股蓝的有效成分转化提供实验依据.
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| 关 键 词： | 绞股蓝 肺癌A549细胞 成分转化 |
A549 Cell Inhibitory Activity from Heat-Processed Gynostemma pentaphyllum |
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| PIAO Xiang-lan,WU Qian,YANG Jing,LIU Hui-min. A549 Cell Inhibitory Activity from Heat-Processed Gynostemma pentaphyllum[J]. Journal of The Central University for Nationalities(Natural Sciences Edition), 2012, 21(1): 49-53 |
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| Authors: | PIAO Xiang-lan WU Qian YANG Jing LIU Hui-min |
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| Affiliation: | (Institute of Chinese Minority Traditional Medicine,Minzu University of China,Beijing 100081,China) |
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| Abstract: | Objective: Comparation of the A549 cell inhibitory activities of the methanol extracts of Gynostemma pentaphyllum before and after heating processing.Method: The leaves of Gynostemma pentaphyllum were steam-heated at 125oC for 3h and 0.24 MPa,and ultra-sonication extracted with methanol for 1h.The methanol extracts of G.pentaphyllum and heat-processed G.pentaphyllum were analyzed by LC/MS and A549 cell inhibition assay tested.Result: Some constituents were transformed by heat-processing from G.pentaphyllum.Heat processed G.pentaphyllum showed significantly A549 cell inhibitory activity.Conclusion: The constituents were transformed by heat-processing and heat processed G.pentaphyllum was enhanced A549 cell inhibitory activity. |
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| Keywords: | Gynostemma pentaphyllum lung cancer A549 cell constituent transformation |
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