| HPLC法测定小儿金翘颗粒中连翘苷的含量 |
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| 引用本文： | 李志成,邓涛,杨永寿.HPLC法测定小儿金翘颗粒中连翘苷的含量[J].大理学院学报,2012,11(6):23-25. |
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| 作者姓名： | 李志成 邓涛 杨永寿 |
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| 作者单位： | 大理学院药学与化学学院,云南大理,671000 |
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| 基金项目： | 大理学院大学生科研基金资助项目 |
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| 摘 要： | 目的:建立小儿金翘颗粒中连翘苷的含量测定方法。方法:采用高效液相色谱法,以0.1%磷酸-乙腈为流动相进行梯度洗脱,流速1.0 mL/min,检测波长277 nm。结果:连翘苷进样量在0.5~5.0μg范围内呈良好的线性关系,r=0.999 9;加样回收率为99.3%,RSD为0.25%(n=6)。结论:该方法准确可靠,重复性好,可用于小儿金翘颗粒的质量控制。
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| 关 键 词： | 连翘苷 小儿金翘颗粒 高效液相色谱法 |
Determination of Forsythin in Xiaoerjinqiao Granules by HPLC |
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| LI Zhicheng,DENG Tao,YANG Yongshou.Determination of Forsythin in Xiaoerjinqiao Granules by HPLC[J].Journal of Dali University,2012,11(6):23-25. |
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| Authors: | LI Zhicheng DENG Tao YANG Yongshou |
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| Institution: | *(College of Pharmacy and Chemistry,Dali University,Dali 671000,China) |
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| Abstract: | Objective: To establish a method to determine the content of Forsythin in Xiaoerjinqiao Granules.Methods: High performance liquid chromatography(HPLC) was applied with the condition of 0.1% phosphoric acid-acetonitrile as mobile phase for gradient elution,the flow rate was 1.0 mL/min,the detection wavelength was 277 nm.Results: The calibration curve was linear in the range of 0.5-5.0 μg for Forsythin,r=0.999 9;the average recovery was 99.3%,and relative standard deviation RSD=0.25%(n=6).Conclusion: The established method is accurate and reliable,and can be used to determine the Forsythin in Xiaoerjinqiao Granules. |
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| Keywords: | Forsythin Xiaoerjinqiao Granules HPLC |
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