| 美洲大蠊提取物对肝星状细胞增殖能力的影响 |
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| 引用本文： | 张小雪,李春艳,徐静,雷占平,段云忠,赖泳.美洲大蠊提取物对肝星状细胞增殖能力的影响[J].大理学院学报,2012,11(6):16-19. |
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| 作者姓名： | 张小雪 李春艳 徐静 雷占平 段云忠 赖泳 |
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| 作者单位： | 大理学院药学与化学学院,云南大理,671000 |
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| 基金项目： | 大理学院大学生科研基金项目 |
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| 摘 要： | 目的:研究美洲大蠊提取物样品Ⅰ、Ⅱ、Ⅲ对体外培养大鼠肝星状细胞株(HSC-T6)增殖的影响。方法:用四甲基偶氮噻唑蓝(MTT)法检测不同浓度样品在不同培养时间作用下HSC-T6细胞的增殖能力。结果:与空白对照组比较,样品Ⅰ抑制了HSC-T6的增殖,且随时间的延长抑制作用加强,在一定的浓度范围内具有剂量依赖性,培养72 h时作用最强。样品Ⅱ与样品Ⅰ作用效果相似,但抑制作用略小于样品Ⅰ。样品Ⅲ在作用24 h时,有一定抑制作用,而在其他时间段基本没有抑制作用。结论:样品Ⅰ、Ⅱ、Ⅲ均能抑制HSC-T6的增殖,有可能成为防治肝纤维化的理想药。
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| 关 键 词： | 肝星状细胞 美洲大蠊提取物 细胞增殖 抑制作用 |
Cell Proliferation Effect of Periplaneta americana Extracts on Hepatic Stellate Cells |
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| ZHANG Xiaoxue,LI Chunyan,XU Jing,LEI Zhanping,DUAN Yunzhong,LAI Yong.Cell Proliferation Effect of Periplaneta americana Extracts on Hepatic Stellate Cells[J].Journal of Dali University,2012,11(6):16-19. |
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| Authors: | ZHANG Xiaoxue LI Chunyan XU Jing LEI Zhanping DUAN Yunzhong LAI Yong |
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| Institution: | * (College of Pharmacy and Chemistry,Dali University,Dali,Yunnan 671000,China) |
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| Abstract: | Objective: To study the proliferation effects of Periplaneta americana extracts(samples Ⅰ,Ⅱ,Ⅲ) on rat hepatic stellate cell lines(HSC-T6) in vitro.Methods: HSC-T6 proliferation under the treatment of Periplaneta americana extracts in different concentration and time was determined by MTT method.Results: Compared with the control group,HSC-T6 proliferation was inhibited by sample Ⅰ with time dependence and reached the peak at 72 hours with concentration dependence within a certain range.Sample Ⅱ showed the same inhibitory pattern but with less effect.Sample Ⅲ showed a particular inhibition effect in 24 hours,and no effect at other time points.Conclusion: The samples Ⅰ,Ⅱ,Ⅲ can inhibit hepatic stellate cell proliferation,which may be a potential drug for prevention and treatment of liver fibrosis. |
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| Keywords: | hepatic stellate cells Periplaneta americana extracts cell proliferation inhibition |
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