| 福氏志贺菌ipaB基因的克隆及序列测定 |
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| 引用本文： | 王国富,白丽,薛士鹏,吴利先.福氏志贺菌ipaB基因的克隆及序列测定[J].大理学院学报,2012,11(3):28-30. |
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| 作者姓名： | 王国富 白丽 薛士鹏 吴利先 |
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| 作者单位： | 大理学院基础医学院,云南大理,671000 |
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| 基金项目： | 云南省教育厅科研基金重点资助项目 |
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| 摘 要： | 目的:克隆福氏志贺菌(Shigella flexneri,S.flexne)的ipaB基因。方法:以福氏志贺菌2a 2457T(Nal)r为模板,用PCR扩增ipaB目的基因片段,克隆至pQE-30表达载体中,构建含目的基因的表达质粒pQE-ipaB。结果:构建了重组质粒pQE-ipaB,ipaB基因全长1 743 bp,经测序分析与预期相符。结论:成功克隆了ipaB基因。
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| 关 键 词： | 福氏志贺菌 ipaB基因 克隆 测定 一致 |
Cloning and Sequencing of IpaB Gene of Shigella flexneri |
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| WANG Guofu,BAI Li,XUE Shipeng,WU Lixian.Cloning and Sequencing of IpaB Gene of Shigella flexneri[J].Journal of Dali University,2012,11(3):28-30. |
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| Authors: | WANG Guofu BAI Li XUE Shipeng WU Lixian |
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| Institution: | (Pre-clinical College,Dali University,Dali,Yunnan 671000,China) |
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| Abstract: | Objective:To clone and sequence of ipaB gene of Shigella flexneri.Methods:IpaB gene was amplified by PCR.The DNA products of ipaB were inserted into a prokaryotic expression vector pQE-30,and then translated into E.coli strain Top10,restriction digestion and sequencing.Results:The recombinant plasmid was constructed and ipaB gene was sequenced as 1 743 bp,conformity with expectation.Conclusion:The results indicated that recombinant plasmid was constructed successfully. |
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| Keywords: | Shigella flexneri ipaB gene cloning assay conformity |
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