炙升麻与几种升麻属植物抗氧化活性研究

目的:了解常见升麻属植物抗氧化活性,探讨中药材升麻炮制过程中酚酸类成分与抗氧化活性的变化。方法:采用DPPH法与HPLC法对8种亚洲、北美洲升麻属植物共计26个样品进行抗氧化活性与酚酸类成分分析,以了解氧化活性与酚酸类成分之间的关系。结果:绝大部分样品IC50>150μg.mL-1;炙升麻在炮制过程中酚酸类成分会发生变化,且工艺流程对结果影响很大,抗氧化活性也会受到进一步影响。结论:升麻属植物样品总体上抗氧化活性不高;中药材升麻植物来源具有多样性,不同的样品间在抗氧化活性方面存在较大差异;炙升麻在炮制过程中酚酸类成分会发生改变,从而导致其抗氧化活性与原植物相比发生较大变化,是否影响升麻的药性与药效值得进一步研究。

Antioxidative Activities of Actaea Plants and Processed Shengma

Objective: To better understand the antioxidative activities of the processed Shengma(Zhi Shengma) as well as the common Actaea plants.Methods: Studies on 26 samples of eight Actaea species from both Asia and North America were performed by using DPPH assay and HPLC analysis.Results: Most of the Actaea samples showed weak/no antioxidative activity with IC50> 150 μg·mL-1.Conclusion: The raw extracts of the roots and rhizomes of the Actaea plants only possessed low antioxidative activities.Actaea species is rich in polyphenols and the latter often show certain antioxidative activities.However,since the chemical profiles for polyphenols of Actaea samples are determined by many factors like plant sources,degree of drying,the way to prepare the processed Shengma,the quality and antioxidative activity of both Shengma and processed Shengma are often unstable.Great care should be taken when Shengma or processed Shengma are subjected for bioactive evaluations.