| 药用甘薯组织培养中酶谱的变化初报 |
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| 引用本文： | 王支槐 李坤培. 药用甘薯组织培养中酶谱的变化初报[J]. 西南师范大学学报(自然科学版), 1992, 17(3): 376-381 |
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| 作者姓名： | 王支槐 李坤培 |
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| 作者单位： | 西南师范大学生物系 重庆630715(王支槐,李坤培,张启堂),西南师范大学生物系 重庆630715(黄继承) |
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| 摘 要： | 药用甘薯—西蒙1号的离体茎、叶及块根外植体,在含有不同配比的植物生长调节剂的MS培养基中可脱分化形成愈伤组织.改变植物生长调节剂的配比,可使其再分化形成根.如切取茎尖或带腋芽的茎段,则可直接形成小植株.西蒙1号茎、叶及块根脱分化形成的愈伤组织及再分化形成的根,其过氧化物酶及淀粉酶同工酶活性,以及谱带数均有所不同,这些变化与其分化状态有关.
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| 关 键 词： | 甘薯 过氧化物 同功酶 组织培养 |
CHANGES OF PEROXIDASE AND AMYLASE ISOZYME IN TISSUE CULTURE OF MEDICINAL SWEET POTATO |
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| Wang Zhihuai Li Kungpei Zhang Qitang Huang Jicheng. CHANGES OF PEROXIDASE AND AMYLASE ISOZYME IN TISSUE CULTURE OF MEDICINAL SWEET POTATO[J]. Journal of southwest china normal university(natural science edition), 1992, 17(3): 376-381 |
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| Authors: | Wang Zhihuai Li Kungpei Zhang Qitang Huang Jicheng |
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| Abstract: | Segments of leaf, stem and tuberous root of medicinal sweet potato "Simon I", cultured in vitro on MS medium supplemented with different plant growth regulators, could be dcdiffcrentiated into calluses. When the ratio of plant growth regulators changed, the root could be produced from the calluses. The plantlcts can be directly produced if the apical part of stem or a piece of stem with axillary buds were applied. The activity and band number of peroxidase and amylase in the calluses deffercntiatcd from the stem, leaf and tuberous root of medicinal sweet potato "Simon 1" and in roots dediffcrentiated from those calluses sahow variations which are more or less related to their different differential states. |
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| Keywords: | Medicinal sweet potato pcroxidasc isozymc amylasc isozyme tissue culture |
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