The involvement of fructose 2,6-bisphosphate in substrate cycle control in the nonoxidative stage of the pentose phosphate pathway. A phosphorus magnetic resonance spectroscopy study |
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| Authors: | N F Belyaeva M A Golubev J A Grigorovich Z V Dubinsky N D Semenova E Pitkänen B F Korovkin |
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| Institution: | (1) Institute of Biological and Medical Chemistry RAMS, Pogodinskaya Str. 10, 119121 Moscow, (Russia);(2) Institute of Chemical Physics RAS, Moscow, (Russia);(3) Faurth Department of Medicine, University Hospital, Helsinki, (Findland) |
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| Abstract: | The role of fructose 2,6-bisphosphate in the interconversion of sedoheptulose 7-phosphate and sedoheptulose 1,7-bisphosphate in rat liver cytosol fractions was studied by means of phosphorus magnetic resonance spectroscopy. When the activit of 6-phosphofructo-1-kinase was inhibited by a high concentration of ATP, the addition of fructose 2,6-bisphosphate led to a marked decrease in sedopheptulsoe 7-phosphate levels, accompanied by an increased concentration of ADP. Frructose 2,6-bisphosphate essentially inhibited both the decrease in sedoheptulose 1,7-disphosphate concentration and the accumulation of Pi in the incubation mixture. The data provided evidence that fructose 2,6-bisphosphate can regulate the substrate cycle; sedoheptulose 7-phosphate  sedoheptulose 1,7-bisphosphate in the liver, and thus control the flux through the nonoxidative stage of the pentose phosphate pathway. |
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| Keywords: | Fructose 2 6-bisphosphate NMR-spectroscopy 6-phosphofructo-1-kinase rat liver sedoheptulose 7-phosphate sedoheptulose 1 7-bisphosphate |
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