| 邻苯三酚红-蛋白质体系的共振光散射光谱研究 |
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| 引用本文： | 吴茂江,刘建平,黎萍.邻苯三酚红-蛋白质体系的共振光散射光谱研究[J].青海师范大学学报(自然科学版),2003(4):52-54. |
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| 作者姓名： | 吴茂江 刘建平 黎萍 |
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| 作者单位： | 1. 宁夏固原师范专科学校,化学系,宁夏,固原,756000
2. 宁夏医学院化学教研室,宁夏,银川,750004 |
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| 摘 要： | 本文研究了邻苯三酚红(PR)与蛋白质的结合反应。在pH3．3的Brium-Robiaon(BR)缓冲溶液中，PR与蛋白质通过分子间作用力形成缔合物，使最大波长约为385nm的共振光散射光谱得以加强。以PR为标记物根据其共振光散射的增强程度，可用于蛋白质的定量测定。对牛血清白蛋白，测定的线性范围为0-5．0mg／L。该方法已用于人尿样品中蛋白质含量的测定。
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| 关 键 词： | 共振光散射 邻苯三酚红 蛋白质 |
| 文章编号： | 1001-7542(2003)04-0052-03 |
| 修稿时间： | 2003年6月28日 |
Resonance light scattering of protein- pyrogallol red system and its analytical application |
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| WU Mao-jiang,LIU Jian-ping,LI Ping.Resonance light scattering of protein- pyrogallol red system and its analytical application[J].Journal of Qinghai Normal University(Natural Science Edition),2003(4):52-54. |
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| Authors: | WU Mao-jiang LIU Jian-ping LI Ping |
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| Institution: | WU Mao-jiang~1,LIU Jian-ping~2,LI Ping~2 |
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| Abstract: | The binding reactions of the pyrogallol red with proteins have been studied.In Britton-Robison buffer of pH 3.3 the pyrogallol red combines with proteins by intermolecular force to form complexes,causing an enhance of resonance light scattering (RLS)at maximum wavelength of about 385nm.Based on the enhancement of RLS,a novel method for the determination of proteins using pyrogallol red as a labeling agent was developed.The linear range for the determination of proteins is 0 to 5.0mg/L.The method has been used to determine proteins in human urine samples with satisfactory results. |
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| Keywords: | resonance light scattering Pyrogallol red protein |
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