| 以魔芋多糖为碳源的产聚羟基丁酸酯菌的筛选、鉴定及发酵研究 |
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| 作者姓名： | 李凌凌 毛伟 张家铭 黄皓 左振宇 杨忠华 |
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| 作者单位： | 武汉科技大学化学与化工学院;武汉科技大学煤转化与新型炭材料湖北省重点实验室 |
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| 基金项目： | 国家自然科学基金资助项目(21376184)；武汉科技大学青年科技骨干培育计划项目(2016XZ014)；国家大学生创新创业训练计划项目(201710488017)；武汉科技大学大学生科技创新基金研究项目(16ZRA044). |
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| 摘 要： | 为充分利用发酵魔芋低聚糖所产生的菌体废弃物,降低微生物发酵法生产聚羟基丁酸酯(PHB)的成本,从云南省种植魔芋的土壤中分离得到一株以魔芋多糖为唯一碳源生长的菌株LKH,该菌可以分泌β-甘露聚糖酶水解魔芋多糖生长,并在胞内积累PHB。经形态学、生理生化特征及16S rDNA序列分析表明,菌株LKH归属于依利诺斯类芽孢杆菌。红外光谱和气相色谱分析结果显示,以魔芋多糖为碳源培养的细菌胞内提取的白色薄膜物质中含有PHB。当氮源(NH_4Cl)浓度为0.2g/L时,菌体以魔芋多糖为碳源合成的PHB质量最高可达0.85g/L,其含量达到69.55%,提取率为93.29%。
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| 关 键 词： | 聚羟基丁酸酯 魔芋多糖 碳源 β-甘露聚糖酶 依利诺斯类芽孢杆菌 微生物发酵 |
| 收稿时间： | 2018/4/17 0:00:00 |
Screening, identification and ferment conditions of polyhydroxybutyrate- producing bacteria with konjac glucomannan as carbon source |
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| Authors: | Li Lingling Mao Wei Zhang Jiaming Huang Hao Zuo Zhenyu and Yang Zhonghua |
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| Institution: | College of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, China;Hubei Coal Conversion and New Carbon Materials Key Laboratory, Wuhan University of Science and Technology, Wuhan 430081, China,College of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, China,College of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, China,College of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, China;Hubei Coal Conversion and New Carbon Materials Key Laboratory, Wuhan University of Science and Technology, Wuhan 430081, China,College of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, China;Hubei Coal Conversion and New Carbon Materials Key Laboratory, Wuhan University of Science and Technology, Wuhan 430081, China and College of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, China;Hubei Coal Conversion and New Carbon Materials Key Laboratory, Wuhan University of Science and Technology, Wuhan 430081, China |
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