EB病毒及化学致癌物DNP对人胚鼻咽粘膜裸鼠移植的诱癌研究

用EBV,DNP及PMA分别或协同对人胚鼻咽粘膜裸鼠移植物进行诱癌研究,探讨上述因素在人实验性鼻咽癌发生中的作用。免疫荧光法检查证实,经B95-8上清液体外及体内转染的人胚鼻咽粘膜上皮表达EBNA。实验结果显示,实验各组均可见各种增生性改变,对照组仅见一般增生性病变。各诱癌组均可见癌前病变,组间无显著性差异。共出现癌8例(1)EBV组:原位癌1例。 (2)EBV+PMA组:早期浸润癌1例。(3)EBV+DNP组:原位癌及浸润癌各1例。(4)EBV+DNP+PMA组:浸润癌1例。 (5)DNP组:原位癌1例。(6)DNP+PMA组:原位癌及浸润癌各1例。结果提示EBV可能在人鼻咽上皮实验性癌变过程中起病因作用。本研究成功诱发了三例浸润癌,均系DAP与其它因素(PMA、EBV)协同作用,提示人鼻咽癌的发生可能与多种致癌因素的相互作用有关。

CARCINOGENESIS OF EPSTEIN-BARR VIRUS AND CHEMICAL CARCINOGEN DNP ON TRANSPLANTS OF HUMAN FETAL NASOPHARYNGEAL MUCOSA IN NUDE MICE

The study presented investigates the separate or corporate carcinogencsis of EBV, DNP and PMA on human fetal nasopharyngeal mucosa transplants in nude mice and effects of the factors above on the formation of human experimental NPC. The results show that the transplant epithelia sucessfully express EBNA in EBV transfected groups. General hyperplasia were observed in control group, whereas precancerous lesions in other experimental groups. 8 cases of carcinomas were found, which included: 4 cases of car, in situ in EBV, DNP, EBV DNP and DNP PMA groups separately, 1 of early infiltrating car.in EBV PMA group, 3 of typical infiltrating car. in EBV DNP, DNP PMA and EBV DNP PMA groups respectively. From the results above, it is estimated that EBV play a role in carcinogenesis of experimental NPC and also suggested that DNP be an important carcinogen in NPC carcinogenesis which is closely related to the corporation of different carcinogenic factors.